1ofx: Difference between revisions

Line 1:

[[Image:1ofx.jpg|left|200px]]<br /><applet load="1ofx" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1ofx.jpg|left|200px]]<br /><applet load="1ofx" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1ofx, resolution 2.000Å" />

'''CRYSTAL STRUCTURE OF AN OKAZAKI FRAGMENT AT 2 ANGSTROMS RESOLUTION'''<br />

==Overview==

In DNA replication, Okazaki fragments are formed as double-stranded, intermediates during synthesis of the lagging strand. They are composed of, the growing DNA strand primed by RNA and the template strand. The DNA, oligonucleotide d(GGGTATACGC) and the chimeric RNA-DNA oligonucleotide, r(GCG)d(TATACCC) were combined to form a synthetic Okazaki fragment and, its three-dimensional structure was determined by x-ray crystallography., The fragment adopts an overall A-type conformation with 11 residues per, turn. Although the base-pair geometry, particularly in the central TATA, part, is distorted, there is no evidence for a transition from the A- to, the B-type conformation at the junction between RNA.DNA hybrid and DNA, duplex. The RNA trimer may, therefore, lock the complete fragment in an, A-type conformation.

In DNA replication, Okazaki fragments are formed as double-stranded intermediates during synthesis of the lagging strand. They are composed of the growing DNA strand primed by RNA and the template strand. The DNA oligonucleotide d(GGGTATACGC) and the chimeric RNA-DNA oligonucleotide r(GCG)d(TATACCC) were combined to form a synthetic Okazaki fragment and its three-dimensional structure was determined by x-ray crystallography. The fragment adopts an overall A-type conformation with 11 residues per turn. Although the base-pair geometry, particularly in the central TATA part, is distorted, there is no evidence for a transition from the A- to the B-type conformation at the junction between RNA.DNA hybrid and DNA duplex. The RNA trimer may, therefore, lock the complete fragment in an A-type conformation.

==About this Structure==

1OFX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with SPM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1OFX OCA].

1OFX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SPM:'>SPM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OFX OCA].

==Reference==

Line 20:

[[Category: double helix]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sun Nov 25 00~~:51:~~56 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:17:22 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1ofx.jpg|left|200px]]<br /><applet load="1ofx" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ofx.jpg|left|200px]]<br /><applet load="1ofx" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1ofx, resolution 2.000&Aring;" />
 '''CRYSTAL STRUCTURE OF AN OKAZAKI FRAGMENT AT 2 ANGSTROMS RESOLUTION'''<br />
 ==Overview==
-In DNA replication, Okazaki fragments are formed as double-stranded, intermediates during synthesis of the lagging strand. They are composed of, the growing DNA strand primed by RNA and the template strand. The DNA, oligonucleotide d(GGGTATACGC) and the chimeric RNA-DNA oligonucleotide, r(GCG)d(TATACCC) were combined to form a synthetic Okazaki fragment and, its three-dimensional structure was determined by x-ray crystallography., The fragment adopts an overall A-type conformation with 11 residues per, turn. Although the base-pair geometry, particularly in the central TATA, part, is distorted, there is no evidence for a transition from the A- to, the B-type conformation at the junction between RNA.DNA hybrid and DNA, duplex. The RNA trimer may, therefore, lock the complete fragment in an, A-type conformation.
+In DNA replication, Okazaki fragments are formed as double-stranded intermediates during synthesis of the lagging strand. They are composed of the growing DNA strand primed by RNA and the template strand. The DNA oligonucleotide d(GGGTATACGC) and the chimeric RNA-DNA oligonucleotide r(GCG)d(TATACCC) were combined to form a synthetic Okazaki fragment and its three-dimensional structure was determined by x-ray crystallography. The fragment adopts an overall A-type conformation with 11 residues per turn. Although the base-pair geometry, particularly in the central TATA part, is distorted, there is no evidence for a transition from the A- to the B-type conformation at the junction between RNA.DNA hybrid and DNA duplex. The RNA trimer may, therefore, lock the complete fragment in an A-type conformation.
 ==About this Structure==
-OFX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with SPM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OFX OCA].
+OFX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SPM:'>SPM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OFX OCA].
 ==Reference==
@@ Line 20: / Line 20: @@
 [[Category: double helix]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:51:56 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:17:22 2008''