1g75: Difference between revisions

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[[Image:1g75.gif|left|200px]]<br /><applet load="1g75" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1g75.gif|left|200px]]<br /><applet load="1g75" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1g75, resolution 1.57Å" />

'''MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGAATF5UCGCG): 5-FORMYLURIDINE/ ADENOSINE BASE-PAIRS IN B-DNA'''<br />

==Overview==

To investigate the role of divalent cations in crystal packing, four, different crystals of a Dickerson-type dodecamer with the sequence, d(CGCGAATXCGCG), containing 2'-deoxy-5-formyluridine at X, were obtained, under several conditions with and without divalent cations. The crystal, structures are all isomorphous. The octahedrally hydrated magnesium, cations found in the major groove cement the two neighbouring duplexes, along the b axis. In the Mg(2+)-free crystals, a five-membered ring of, water molecules occupies the same position as the magnesium site and, connects the two duplexes similarly to the hydrated Mg(2+) ion. It has, been concluded that water molecules can take the place of the hydrated, magnesium cation in crystallization, but the magnesium cation is more, effective and gives X-ray diffraction at slightly higher resolution. In, all four crystals, the 5-formyluracil residues form the canonical, Watson-Crick pair with adenine residues.

To investigate the role of divalent cations in crystal packing, four different crystals of a Dickerson-type dodecamer with the sequence d(CGCGAATXCGCG), containing 2'-deoxy-5-formyluridine at X, were obtained under several conditions with and without divalent cations. The crystal structures are all isomorphous. The octahedrally hydrated magnesium cations found in the major groove cement the two neighbouring duplexes along the b axis. In the Mg(2+)-free crystals, a five-membered ring of water molecules occupies the same position as the magnesium site and connects the two duplexes similarly to the hydrated Mg(2+) ion. It has been concluded that water molecules can take the place of the hydrated magnesium cation in crystallization, but the magnesium cation is more effective and gives X-ray diffraction at slightly higher resolution. In all four crystals, the 5-formyluracil residues form the canonical Watson-Crick pair with adenine residues.

==About this Structure==

1G75 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with K and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1G75 OCA].

1G75 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=K:'>K</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G75 OCA].

==Reference==

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[[Category: modified nucleotide]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sun Nov 25 00~~:57:~~21 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:46:53 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1g75.gif|left|200px]]<br /><applet load="1g75" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1g75.gif|left|200px]]<br /><applet load="1g75" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1g75, resolution 1.57&Aring;" />
 '''MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGAATF5UCGCG): 5-FORMYLURIDINE/ ADENOSINE BASE-PAIRS IN B-DNA'''<br />
 ==Overview==
-To investigate the role of divalent cations in crystal packing, four, different crystals of a Dickerson-type dodecamer with the sequence, d(CGCGAATXCGCG), containing 2'-deoxy-5-formyluridine at X, were obtained, under several conditions with and without divalent cations. The crystal, structures are all isomorphous. The octahedrally hydrated magnesium, cations found in the major groove cement the two neighbouring duplexes, along the b axis. In the Mg(2+)-free crystals, a five-membered ring of, water molecules occupies the same position as the magnesium site and, connects the two duplexes similarly to the hydrated Mg(2+) ion. It has, been concluded that water molecules can take the place of the hydrated, magnesium cation in crystallization, but the magnesium cation is more, effective and gives X-ray diffraction at slightly higher resolution. In, all four crystals, the 5-formyluracil residues form the canonical, Watson-Crick pair with adenine residues.
+To investigate the role of divalent cations in crystal packing, four different crystals of a Dickerson-type dodecamer with the sequence d(CGCGAATXCGCG), containing 2'-deoxy-5-formyluridine at X, were obtained under several conditions with and without divalent cations. The crystal structures are all isomorphous. The octahedrally hydrated magnesium cations found in the major groove cement the two neighbouring duplexes along the b axis. In the Mg(2+)-free crystals, a five-membered ring of water molecules occupies the same position as the magnesium site and connects the two duplexes similarly to the hydrated Mg(2+) ion. It has been concluded that water molecules can take the place of the hydrated magnesium cation in crystallization, but the magnesium cation is more effective and gives X-ray diffraction at slightly higher resolution. In all four crystals, the 5-formyluracil residues form the canonical Watson-Crick pair with adenine residues.
 ==About this Structure==
-G75 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with K and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G75 OCA].
+G75 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=K:'>K</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G75 OCA].
 ==Reference==
@@ Line 26: / Line 26: @@
 [[Category: modified nucleotide]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:57:21 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:46:53 2008''