1wza: Difference between revisions
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New page: left|200px<br /><applet load="1wza" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wza, resolution 1.60Å" /> '''Crystal structure of... |
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[[Image:1wza.gif|left|200px]]<br /><applet load="1wza" size=" | [[Image:1wza.gif|left|200px]]<br /><applet load="1wza" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1wza, resolution 1.60Å" /> | caption="1wza, resolution 1.60Å" /> | ||
'''Crystal structure of alpha-amylase from H.orenii'''<br /> | '''Crystal structure of alpha-amylase from H.orenii'''<br /> | ||
==Overview== | ==Overview== | ||
Here we report the first crystal structure of a protein, AmyA, a secretory | Here we report the first crystal structure of a protein, AmyA, a secretory alpha-amylase isolated from Halothermothrix orenii, which is both halophilic and thermophilic. The crystal structure was determined at 1.6 A resolution. AmyA lacks the conserved acidic surface, which is considered essential for protein stability at high salinity. Sedimentation velocity and CD experiments on AmyA reveal the formation of unique reversible poly-dispersed oligomers that show unusually high thermal stability. These studies provide valuable insight into the structural elements that contribute to the stability of AmyA at both physical and chemical extremes and their functional implications. | ||
==About this Structure== | ==About this Structure== | ||
1WZA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Halothermothrix_orenii Halothermothrix orenii] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] Full crystallographic information is available from [http:// | 1WZA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Halothermothrix_orenii Halothermothrix orenii] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WZA OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: thermophilic]] | [[Category: thermophilic]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:49:36 2008'' | ||
