1st8: Difference between revisions
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New page: left|200px<br /><applet load="1st8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1st8, resolution 2.35Å" /> '''Crystal structure of... |
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[[Image:1st8.gif|left|200px]]<br /><applet load="1st8" size=" | [[Image:1st8.gif|left|200px]]<br /><applet load="1st8" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1st8, resolution 2.35Å" /> | caption="1st8, resolution 2.35Å" /> | ||
'''Crystal structure of fructan 1-exohydrolase IIa from Cichorium intybus'''<br /> | '''Crystal structure of fructan 1-exohydrolase IIa from Cichorium intybus'''<br /> | ||
==Overview== | ==Overview== | ||
Fructan 1-exohydrolase, an enzyme involved in fructan degradation, belongs | Fructan 1-exohydrolase, an enzyme involved in fructan degradation, belongs to the glycosyl hydrolase family 32. The structure of isoenzyme 1-FEH IIa from Cichorium intybus is described at a resolution of 2.35 A. The structure consists of an N-terminal fivefold beta-propeller domain connected to two C-terminal beta-sheets. The putative active site is located entirely in the beta-propeller domain and is formed by amino acids which are highly conserved within glycosyl hydrolase family 32. The fructan-binding site is thought to be in the cleft formed between the two domains. The 1-FEH IIa structure is compared with the structures of two homologous but functionally different enzymes: a levansucrase from Bacillus subtilis (glycosyl hydrolase family 68) and an invertase from Thermotoga maritima (glycosyl hydrolase family 32). | ||
==About this Structure== | ==About this Structure== | ||
1ST8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cichorium_intybus Cichorium intybus] with GOL as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Fructan_beta-fructosidase Fructan beta-fructosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.80 3.2.1.80] Full crystallographic information is available from [http:// | 1ST8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cichorium_intybus Cichorium intybus] with <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Fructan_beta-fructosidase Fructan beta-fructosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.80 3.2.1.80] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ST8 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Fructan beta-fructosidase]] | [[Category: Fructan beta-fructosidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Ende, W | [[Category: Ende, W Van den.]] | ||
[[Category: Laere, A | [[Category: Laere, A Van.]] | ||
[[Category: Rabijns, A.]] | [[Category: Rabijns, A.]] | ||
[[Category: Ranter, C | [[Category: Ranter, C J.De.]] | ||
[[Category: Verhaest, M.]] | [[Category: Verhaest, M.]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: five fold beta propeller]] | [[Category: five fold beta propeller]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:04:56 2008'' | ||
