Extremophiles: Difference between revisions
From Proteopedia
Jump to navigationJump to search
mNo edit summary |
No edit summary |
||
| Line 3: | Line 3: | ||
Proteins of thermophiles and halophiles tend to be more stable, easier to manage in the laboratory, and easier to crystallize than proteins from mesophiles. Consequently, extremophile proteins have been very useful in crystallography and [[Structural genomics|structural genomics]], yielding many new structures<ref>PMID: 17563834</ref><ref>PMID: 19156357</ref>. For example, the [[Nobel_Prizes_for_3D_Molecular_Structure|Nobel Prize-winning]] structures of the [[Ribosome|ribosome]] were determined using proteins from the thermophile ''Thermus thermophilus'' and the halophile ''Haloarcula marismortui'' (see 2009 at [[Nobel Prizes for 3D Molecular Structure#Twenty-First_Century]]). | Proteins of thermophiles and halophiles tend to be more stable, easier to manage in the laboratory, and easier to crystallize than proteins from mesophiles. Consequently, extremophile proteins have been very useful in crystallography and [[Structural genomics|structural genomics]], yielding many new structures<ref>PMID: 17563834</ref><ref>PMID: 19156357</ref>. For example, the [[Nobel_Prizes_for_3D_Molecular_Structure|Nobel Prize-winning]] structures of the [[Ribosome|ribosome]] were determined using proteins from the thermophile ''Thermus thermophilus'' and the halophile ''Haloarcula marismortui'' (see 2009 at [[Nobel Prizes for 3D Molecular Structure#Twenty-First_Century]]). | ||
Most applications of the polymerase chain reaction (PCR) method now rely on thermostable Taq Polymerase an enzyme from ''Thermus aquauatics'', see [[1taq]], [[1tau]], [[1bgx]]; Kary Mullis | Most applications of the polymerase chain reaction (PCR) method now rely on thermostable Taq Polymerase an enzyme from ''Thermus aquauatics'', see [[1taq]], [[1tau]], [[1bgx]]; Kary Mullis sahred the [http://nobelprize.org/nobel_prizes/chemistry/laureates/1993/ 1993 Nobel Prize in Chemistry] for the invention of PCR. | ||
Even before study and applied use of the extremeophile's enzymes in the lab, the analysis of these diverse organisms had a profound effect on how scientist thought about evolution of life on on our planet and phylogenetic relationships, perhaps best illustrated by [http://www.ucmp.berkeley.edu/archaea/archaea.html Carl Woese's work to classify life into three Domains]: Archaea, Bacteria, and Eukaryota. | Even before study and applied use of the extremeophile's enzymes in the lab, the analysis of these diverse organisms had a profound effect on how scientist thought about evolution of life on on our planet and phylogenetic relationships, perhaps best illustrated by [http://www.ucmp.berkeley.edu/archaea/archaea.html Carl Woese's work to classify life into three Domains]: Archaea, Bacteria, and Eukaryota. | ||
