Net charge: Difference between revisions
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In a solution of a given pH, a protein will have a negative or positive net charge. The [[isoelectric point]], or pI, is the pH at which a protein has zero net charge. When the pH is higher than the isoelectric point, the protein has negative net charge, and when lower, positive net charge. | In a solution of a given pH, a protein will have a negative or positive net charge dependent on the pKa values of the acidic and basic groups of the constiuent amino acids. The [[isoelectric point]], or pI, is the pH at which a protein has zero net charge. When the pH is higher than the isoelectric point, the protein has negative net charge, and when lower, positive net charge. | ||
<br>You can calculate the net charge of your protein easily at a given pH or over a range: | <br>You can calculate the net charge of your protein easily at a given pH or over a range: | ||
