Beta-glucosidase: Difference between revisions
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The enzymatic hydrolysis of a glycosidic bond requires two critical residues : a proton donor and a proton acceptor which can also be called a nucleophile/base. Aspartate and glutamate have been found to perform catalysis [x]. Accorded to this, studies showed that one of the conserved regions of β-glucosidases is centred on conserved glutamate residues [y]. | The enzymatic hydrolysis of a glycosidic bond requires two critical residues : a proton donor and a proton acceptor which can also be called a nucleophile/base. Aspartate and glutamate have been found to perform catalysis [x]. Accorded to this, studies showed that one of the conserved regions of β-glucosidases is centred on conserved glutamate residues [y]. | ||
As every β-glucosidase, 2VRJ presents two conserved residues of glutamate (<scene name='Sandbox_155/166_and_351/4'>166 and 351</scene>). Moreover 2VRJ has a third important residue : <scene name='Sandbox_155/293/2'>asparagin 293</scene>. | As every β-glucosidase, 2VRJ presents two conserved residues of glutamate (<scene name='Sandbox_155/166_and_351/4'>166 and 351</scene>). Moreover 2VRJ has a third important residue : <scene name='Sandbox_155/293/2'>asparagin 293</scene>. | ||
The protein is presented in complex with an inhibitor called <scene name='Sandbox_155/Calystegine/1'>calystegine</scene>. We can see that the two '''<font color='#5CB8D1'>glutamate</font>''' residues and the asparagin are really closed to each other and to the ligand. Such a proximity highly suggests that there are important interactions between them. | The protein is presented in complex with an inhibitor called <scene name='Sandbox_155/Calystegine/1'>calystegine</scene>. We can see that the two '''<font color='#5CB8D1'>glutamate</font>''' residues and the asparagin are really closed to each other and to the ligand (<scene name='Sandbox_155/Ligand_and_residues/1'>see</scene>). Such a proximity highly suggests that there are important interactions between them. | ||
===Hydrolysis of terminal non-reducing residues in β-glucosides=== | ===Hydrolysis of terminal non-reducing residues in β-glucosides=== | ||
