Sandbox122: Difference between revisions
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Blue light arrives on the only LOV2 <scene name='Sandbox122/Cystein_residue/1'>cystein residue</scene>(situate 4.2A from atom C(4a)) and induces formation of covalent cysteinyl-C(4a)adduct. (To explain that, refer to the scheme). Somes studies showed that temperature have an influence on that adduct. In fact, at low temperature, the microenvironment determine the reactivity of the S-H group of Cys966. A base-catalyzed mechanism for dark state recovery. Imidazole snatch proton from N5 atom of FMN and give it to the cysteine again (explain on the scheme). | Blue light arrives on the only LOV2 <scene name='Sandbox122/Cystein_residue/1'>cystein residue</scene>(situate 4.2A from atom C(4a)) and induces formation of covalent cysteinyl-C(4a)adduct. (To explain that, refer to the scheme). Somes studies showed that temperature have an influence on that adduct. In fact, at low temperature, the microenvironment determine the reactivity of the S-H group of Cys966. A base-catalyzed mechanism for dark state recovery. Imidazole snatch proton from N5 atom of FMN and give it to the cysteine again (explain on the scheme). | ||
==LOV2 signal transmission== | ==LOV2 signal transmission== | ||
studies have | Some studies have showed a role for the central β-sheet in propagating the signal generated within the FMN-protein changes at the LOV2 surface, which are necessary for activation of the C-terminal kinase domain.Specifically, the side chain of a conserved glutamine residue within LOV2 (Gln575 in Arabidopsis phot1) which forms hydrogen bonds with the FMN chromophore flips by 180° upon cysteinyl adduct formation causing protein changes in the central β-sheet that forms contacts with the Jα-helix. | ||
This complex may interact directly with the kinase and regulate his activity. | This complex may interact directly with the kinase and regulate his activity. | ||
==Kinase regulation by LOV2== | ==Kinase regulation by LOV2== | ||
