Sandbox122: Difference between revisions
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==LOV2 signal transmission== | ==LOV2 signal transmission== | ||
Some studies have showed a role for the central β-sheet in propagating the signal generated within the FMN-protein changes at the LOV2 surface, which are necessary for activation of the C-terminal kinase domain.Specifically, the side chain of a conserved glutamine residue within LOV2 (Gln575 in Arabidopsis phot1) which forms hydrogen bonds with the FMN chromophore flips by 180° upon cysteinyl adduct formation causing protein changes in the central β-sheet that forms contacts with the Jα-helix. | Some studies have showed a role for the central β-sheet in propagating the signal generated within the FMN-protein changes at the LOV2 surface, which are necessary for activation of the C-terminal kinase domain.Specifically, the side chain of a conserved glutamine residue within LOV2 (Gln575 in Arabidopsis phot1) which forms hydrogen bonds with the FMN chromophore flips by 180° upon cysteinyl adduct formation causing protein changes in the central β-sheet that forms contacts with the Jα-helix. | ||
==Kinase regulation by LOV2== | ==Kinase regulation by LOV2== | ||
The current view of phototropin receptor activation is that LOV2 functions as a repressor of the C-terminal kinase domain in the dark and that this mode of repression is alleviated upon photoexcitation, resulting in receptor autophosphorylation.The predominant role of LOV2 in regulating phototropin activity appears to arise from its position within the phototropin molecule. Photoexcitation of an extended LOV2 fragment leads to displacement of an α-helix from the surface of the LOV2-core. | The current view of phototropin receptor activation is that LOV2 functions as a repressor of the C-terminal kinase domain in the dark and that this mode of repression is alleviated upon photoexcitation, resulting in receptor autophosphorylation.The predominant role of LOV2 in regulating phototropin activity appears to arise from its position within the phototropin molecule. Photoexcitation of an extended LOV2 fragment leads to displacement of an α-helix from the surface of the LOV2-core. | ||
