DNA Polymerase I: Difference between revisions
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The X-ray structure is that of an editing complex, that is, the 3' end of the primer strand, the end that is elongated by the polymerase, occupies the 3'→5' exonuclease active site. This is more clearly seen in a <scene name='Sandbox_dvoet/DNA_polymerase/Klenow-dna-closeup/4'>closeup of the DNA</scene> in which the the rods connecting successive P atoms have been removed for clarity. Note that the base pair closest to the polymerase active site, a G·C, has opened up to enable the 3' end of the primer strand to reach the exonuclease active site. Click here to <scene name='Sandbox_dvoet/DNA_polymerase/Dna-closeup/3'>hide the protein</scene>. | The X-ray structure is that of an editing complex, that is, the 3' end of the primer strand, the end that is elongated by the polymerase, occupies the 3'→5' exonuclease active site. This is more clearly seen in a <scene name='Sandbox_dvoet/DNA_polymerase/Klenow-dna-closeup/4'>closeup of the DNA</scene> in which the the rods connecting successive P atoms have been removed for clarity. Note that the base pair closest to the polymerase active site, a G·C, has opened up to enable the 3' end of the primer strand to reach the exonuclease active site. Click here to <scene name='Sandbox_dvoet/DNA_polymerase/Dna-closeup/3'>hide the protein</scene>. | ||
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==Structure of the whole ''Thermus aquaticus'' DNA polymerase I== | ==Structure of the whole ''Thermus aquaticus'' DNA polymerase I== | ||
<applet load='1taq.pdb' size='450' frame='true' align='right' caption='Thermus aquaticus Pol I' scene= 'Sandbox_dvoet/DNA_polymerase/Taq_pol_i/1'/> | <applet load='1taq.pdb' size='450' frame='true' align='right' caption='Thermus aquaticus Pol I' scene= 'Sandbox_dvoet/DNA_polymerase/Taq_pol_i/1'/> | ||
