Group:SMART:2010 Pingry SMART Team: Difference between revisions

Revision as of 20:11, 8 February 2010 view source Caryn Ha (talk \| contribs) 72 edits →Cofactor specificity ← Older edit		Revision as of 20:19, 8 February 2010 view source Caryn Ha (talk \| contribs) 72 edits →PDB ID: 1m9h, Mutant 2,5-diketo-d-gluconic acid reductase with NADH (mutant) Newer edit →
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	Lys 232 in the 2,5-DKGR wildtype interacts directly with the pyrophosphate group of NADPH through hydrogen bonds. However, in the 2,5-DKGR mutant,this residue has been altered into a <scene name='2010_Pingry_SMART_Team/1m9h_original/2'>Lys232Gly mutation</scene>		Lys 232 in the 2,5-DKGR wildtype interacts directly with the pyrophosphate group of NADPH through hydrogen bonds. However, in the 2,5-DKGR mutant,this residue has been altered into a <scene name='2010_Pingry_SMART_Team/1m9h_original/19'>Lys232Gly mutation</scene>
	to adapt to the absent pyrophosphate group in NADH. Significantly, Gly lacks a side chain since no interaction is necessary due to the absent phosphate group in NADH. In addition, the mutation results in the reduction of cofactor Ka and kcat.		to adapt to the absent pyrophosphate group in NADH. Significantly, Gly lacks a side chain since no interaction is necessary due to the absent phosphate group in NADH. In addition, the mutation results in the reduction of cofactor Ka and kcat.

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