Retroviral Integrase: Difference between revisions
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==Introduction== | ==Introduction== | ||
Integrase is an essential retroviral enzyme that binds to viral DNA and inserts it into a host cell chromosome. [The reverse transcribed cDNA of human immunodeficiency virus type 1 (HIV-1) is inserted in the host cell genome in order increase pathogen fitness and virulence.]It is produced by a class of retrovirus (like HIV) and is used by the virus to incorporate its genetic material into the host cell DNA. The host cellular machinery then produces mRNA and then protein from the incorporated genetic material, thus replicating the virus. Although several integrase inhibiting drugs have been investigated, the mechanism [responsible for strand-transfer inhibition action remain unclear] of action was not clear. On February 1, 2010, a crystal structure of the PFV Intasome was published, paving the way for better retroviral treatments. [However, Hare ''et al'' (2010) determined the structural constituents of retroviral integration. Further elucidation of the complete structure of the retroviral integrase, and its application to regulate functional and enzymatic activities could potentially enable researchers to delay the progression of retroviral diseases. Moreover, study of HIV-1 integration could lead to a promising new target, and contribute to the generation pharmacophore models for antiviral therapy. ] | Integrase is an essential retroviral enzyme that binds to viral DNA and inserts it into a host cell chromosome. [The reverse transcribed cDNA of human immunodeficiency virus type 1 (HIV-1) is inserted in the host cell genome in order increase pathogen fitness and virulence.]It is produced by a class of retrovirus (like HIV) and is used by the virus to incorporate its genetic material into the host cell DNA. The host cellular machinery then produces mRNA and then protein from the incorporated genetic material, thus replicating the virus. Although several integrase inhibiting drugs have been investigated, the mechanism [responsible for strand-transfer inhibition action remain unclear] of action was not clear. On February 1, 2010, a crystal structure of the PFV Intasome was published, paving the way for better retroviral treatments. [However, Hare ''et al'' (2010) determined the structural constituents of retroviral integration. Further elucidation of the complete structure of the retroviral integrase, and its application to regulate functional and enzymatic activities could potentially enable researchers to delay the progression of retroviral diseases. Moreover, study of HIV-1 integration could lead to a promising new target, and contribute to the generation pharmacophore models for antiviral therapy. ] | ||
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==Mechanism for strand-transfer inhibition action== | ==Mechanism for strand-transfer inhibition action== | ||
==References== | ==References== | ||
