Glyceraldehyde-3-Phosphate Dehydrogenase: Difference between revisions
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The mechanism of the glycolysis reaction is fairly straight forward. After the aldehyde enters the <scene name='Nathan_Line_sandbox_3/Active_site/1'>active site</scene>, the sulfhydryl group from <scene name='Nathan_Line_sandbox_3/Cystine/4'>Cystine 151</scene> attacks the nucleophilic carbon to form a thiohemiacetal. This intermediate undergoes oxidation due to a hydride transfer to a nearby NAD+ forming a thioester. From here, a phosphate group enters and attacks the same carbonyl while at the same time it is separated from the cystine by the protonated <scene name='Nathan_Line_sandbox_3/Histidine/2'>Histidine 176</scene> group. This produces the desired 1,3-bisphosphoglycerate. | The mechanism of the glycolysis reaction is fairly straight forward. After the aldehyde enters the <scene name='Nathan_Line_sandbox_3/Active_site/1'>active site</scene>, the sulfhydryl group from <scene name='Nathan_Line_sandbox_3/Cystine/4'>Cystine 151</scene> attacks the nucleophilic carbon to form a thiohemiacetal. This intermediate undergoes oxidation due to a hydride transfer to a nearby NAD+ forming a thioester. From here, a phosphate group enters and attacks the same carbonyl while at the same time it is separated from the cystine by the protonated <scene name='Nathan_Line_sandbox_3/Histidine/2'>Histidine 176</scene> group. This produces the desired 1,3-bisphosphoglycerate. | ||
References: | |||
1) Voet, D, Voet, J, & Pratt, C. (2008). Fundamentals of biochemistry, third edition. Hoboken, NJ: Wiley & Sons, Inc. | |||
2) | |||
