User:Laura Carbone/Sandbox 1: Difference between revisions
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====GFP Mutants Used In FRET==== | ====GFP Mutants Used In FRET==== | ||
<applet load='1ema' size='400' frame='true' align='center' scene='User:Laura_Carbone/Sandbox_1/Chromophore/5' name='5'/> | |||
(Applet is currently showing the chromophore of wild GFP.) | |||
*GFP (eGFP): contains a mutation at Ser<sup>65</sup> in which the residue is most commonly replaced by Thr, Ala, or Gly. Lacks the 395 nm excitation peak, but still emits light at 508 nm like wild-type GFP. | *GFP (eGFP): contains a mutation at Ser<sup>65</sup> in which the residue is most commonly replaced by Thr, Ala, or Gly. Lacks the 395 nm excitation peak, but still emits light at 508 nm like wild-type GFP. | ||
*BFP ([[1bfp]]): blue-shifted GFP that has replaced Tyr<sup>66</sup> with His (Y66H mutation). | *<scene name='User:Laura_Carbone/Sandbox_1/Bfp_chromophore/1'>BFP</scene> ([[1bfp]]): blue-shifted GFP that has replaced Tyr<sup>66</sup> with His (Y66H mutation). | ||
*CFP ([[1oxd]]): contains Y66H mutation with an emission spectra between BFP and eGFP. | *CFP ([[1oxd]]): contains Y66H mutation with an emission spectra between BFP and eGFP. | ||
*Sapphire: excitation spectra at 495 nm has been suppressed while the 395 nm spectra is retained; still emits light at 508 nm. | *Sapphire: excitation spectra at 495 nm has been suppressed while the 395 nm spectra is retained; still emits light at 508 nm. | ||
