Sandbox 181: Difference between revisions
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Glutathione reductase, also known as GSH reductase, converts oxidized glutathione ( | Glutathione reductase, also known as GSH reductase, converts oxidized glutathione (GSSG) to two molecules of reduced [[gluthatione]] (GSH). | ||
== Structure == | == Structure == | ||
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== Reaction == | == Reaction == | ||
The action of glutathione reductase proceeds through a cyclic series of structures in differing redox states (Figure 1). | The action of glutathione reductase proceeds through a cyclic series of structures in differing redox states (Figure 1). NADH binds causing a transient reduction of flavin and this reduced flavin consequently reduces Cys58-Cys63 disulfide bond, forming a short lived covalent intermediate with Cys63. Following this, a stable charge-transfer complex between flavin and the Cys63 thiolate. After this stable charge-transfer complex is formed the NADP+ dissociates and is replaced by another NADPH. This is the end of the reductive first half of the mechanism and the oxidative half is initiated upon the binding of GSSG. | ||
