Isocitrate dehydrogenase: Difference between revisions
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=='''The Active Site'''== | =='''The Active Site'''== | ||
The active site of Isocitrate dehydrogenase binds the NAD+ or an NADP+ molecule as well as an | The active site of Isocitrate dehydrogenase binds the NAD+ or an NADP+ molecule as well as an Mn+ or Ca2+. This metal ion seems to be essential for catalysis<ref>PMID:15173171.</ref>. Side chains of Asp 279 form hydrogen bonds with Ser94 (Human isocitrate dehydrogenase). Isocitrate is able to bind to the active site using about 8 amino acids like tyrosine, serine, asparagine, arginine, arginine, arginine, tyrosine, and lysine<ref>PMID:17632124.</ref>. Across species these are not perfectly conserved by they are replaced with residues of similar properties and also are found in similar areas of the protein. Below is a picture of Porcine Active site with all of its residuals and ligands. | ||
[[Image:A.jpg]] | |||
The blue is the Mn+ ion next to the ligand which is surrounded | |||
by the residuals of that active site<ref>http://en.wikipedia.org/wiki/Isocitrate_dehydrogenase#cite_note-nfr154197.2F32-6</ref>. | |||
=='''References'''== | |||
<references/> | |||
