Sandbox 160: Difference between revisions
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== Introduction == | == Introduction == | ||
{{STRUCTURE_1vc2| PDB=1vc2 | Scene =Sandbox_160/Newscene/1'>active site }} | |||
Glyceraldehyde 3-Phosphate dehydrogenase (GAPDH) is an Oxidoreductase enzyme and is involved in many important biochemical reactions. This protein is responsible for catalyzing the conversion of glyceraldeyde 3-Phosphate into 1,3-Biphosphoglycerate in a two step coupled mechanism. This conversion occurs during step 6 or the beginning of the "payoff phase" of glycolysis (the second half of the entire process) in which ATP and NADH is produced. A total of 2 NADH and 4 ATP are produced during this phase for a net gain of 2 NADH and 2 ATP for the entire glycolysis pathway per glucose. | Glyceraldehyde 3-Phosphate dehydrogenase (GAPDH) is an Oxidoreductase enzyme and is involved in many important biochemical reactions. This protein is responsible for catalyzing the conversion of glyceraldeyde 3-Phosphate into 1,3-Biphosphoglycerate in a two step coupled mechanism. This conversion occurs during step 6 or the beginning of the "payoff phase" of glycolysis (the second half of the entire process) in which ATP and NADH is produced. A total of 2 NADH and 4 ATP are produced during this phase for a net gain of 2 NADH and 2 ATP for the entire glycolysis pathway per glucose. | ||
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Once Glyceraldehyde 3-phophate comes into contact with the active site it forms a hydrogen bond through its C2 hydroxyl group to Cys149N. The C1 hydroxyl group of the substrate binds to His176NE2. Additional hydrogen bonds to the phosphate group of the substrate from additional residues such as Thr1790G1,Arg231NH1 along with N7N and 02'N if the NAD+ moeity help stabilize the molecule during the course of the reaction in the active site. The nicotinamide ring of the NAD+ ligand is responsible for orienting the hydrogen atom at C1 towards itself which allows for easier transfer in producing in reducing NAD+ to NADH. | Once Glyceraldehyde 3-phophate comes into contact with the active site it forms a hydrogen bond through its C2 hydroxyl group to Cys149N. The C1 hydroxyl group of the substrate binds to His176NE2. Additional hydrogen bonds to the phosphate group of the substrate from additional residues such as Thr1790G1,Arg231NH1 along with N7N and 02'N if the NAD+ moeity help stabilize the molecule during the course of the reaction in the active site. The nicotinamide ring of the NAD+ ligand is responsible for orienting the hydrogen atom at C1 towards itself which allows for easier transfer in producing in reducing NAD+ to NADH. | ||
