Sandbox 156: Difference between revisions
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The general structure of CAT III is dominated by a six stranded antiparallel <scene name='Sandbox_156/Scene_4/4'>β-sheet</scene> and 5 <scene name='Sandbox_156/Scene_4/3'>α-helices</scene> stacked against the ends and face of the protein, forming a structure known as an "open-faced sandwhich". <ref>PMID: 3288984</ref> | The general structure of CAT III is dominated by a six stranded antiparallel <scene name='Sandbox_156/Scene_4/4'>β-sheet</scene> and 5 <scene name='Sandbox_156/Scene_4/3'>α-helices</scene> stacked against the ends and face of the protein, forming a structure known as an "open-faced sandwhich". <ref>PMID: 3288984</ref> | ||
===The Chloramphenicol Binding Site=== | |||
The <scene name='Sandbox_156/Scene_3/1'>active site</scene> of CAT III is lined with generally hydrophobic residues, allowing only 2 hydrogen bonds with the substrate. A third hydrogen bond is mediated through a <scene name='Sandbox_156/Scene_4/2'>bridging hydrogen bond</scene> between the the 1-hydroxyl of chloramphenicol and the hydroxyl of tyrosine-174 by a water molecule. | |||
{{STRUCTURE_4CLA | PDB=4CLA | Scene=Sandbox_156/Scene_1/1}} | {{STRUCTURE_4CLA | PDB=4CLA | Scene=Sandbox_156/Scene_1/1}} | ||
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<scene name='Sandbox_156/Scene_4/2'>bridging hydrogen bond</scene> | <scene name='Sandbox_156/Scene_4/2'>bridging hydrogen bond</scene> | ||
<scene name='Sandbox_156/Scene_4/1'>Tyr-25</scene> | <scene name='Sandbox_156/Scene_4/1'>Tyr-25</scene> | ||
