Ubiquitin Structure & Function: Difference between revisions
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[[Image:1ubiq.png|300 px|right]] | [[Image:1ubiq.png|300 px|right]] | ||
=Function= | =Function= | ||
At first, ubiquitin was believed to be a hormone involed in inducing the differentiation of lymphocytes and activating adenylate cyclase <ref>Vijay-Kumar, S., Begg, CE., Wilkinson, KD and Cook, WJ. 1985. Three-dimensional structure of ubiquitin of 2.8 angstrom resolution. ''Proc Natl Acad Sci'' 82:3582-3585</ref>. However, today, ubiquitin is primarily known for its role in intracellular ATP-dependent protein degradation. This is accomplished through the process of | At first, ubiquitin was believed to be a hormone involed in inducing the differentiation of lymphocytes and activating adenylate cyclase <ref>Vijay-Kumar, S., Begg, CE., Wilkinson, KD and Cook, WJ. 1985. Three-dimensional structure of ubiquitin of 2.8 angstrom resolution. ''Proc Natl Acad Sci'' 82:3582-3585</ref>. However, today, ubiquitin is primarily known for its role in intracellular ATP-dependent protein degradation. This is accomplished through the process of several seperate reactions: | ||
=== Activation === | === Activation === | ||
The first step of ubiquitin activation involves the formation of a ubiqiotin-adenylate intermediate. This reaction requires an E1 ubiquitin-activating enzyme. The second step of ubiquitin activation transfers ubiquitin to the E1 active site cysteine residue and AMP is released. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. | |||
=== Ubiquitin Conjugation === | |||
=== Proofreading === | |||
=== Conjugate Metabolism === | |||
[[Image:diubq.png|500 px|center]] | [[Image:diubq.png|500 px|center]] | ||
[[Image:Ubq_pathway.png|500 px|center]] | [[Image:Ubq_pathway.png|500 px|center]] | ||
<references/> | <references/> | ||
