Phosphoglucoisomerase: Difference between revisions
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=='''Mechanism'''== | =='''Mechanism'''== | ||
The proposed reaction mechanism of PGI for the conversion of glucose-6-phosphate to fructose 6-phosphate involves an acid/base catalysis by the enzyme.<ref> Voet D, Voet J, and Pratt C. Fundamentals of Biochemistry Life at the Molecular Level. New York: John Wiley & Sons, 2008. Print.</ref> | The proposed reaction mechanism of PGI for the reversible conversion of glucose-6-phosphate to fructose 6-phosphate involves an acid/base catalysis by the enzyme.<ref> Voet D, Voet J, and Pratt C. Fundamentals of Biochemistry Life at the Molecular Level. New York: John Wiley & Sons, 2008. Print.</ref> | ||
'''Step 1''' The substrate binds to the enzyme. | '''Step 1''' The substrate binds to the enzyme. | ||
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[[Image:Mech.png]] | [[Image:Mech.png]] | ||
'''Regulation'''- it was found that PGI is regulated only by the relative concentrations of glucose-6-phosphate and fructose 6-phosphate, towards equilibrium. Nevertheless, it was found that the kinetic parameters of | '''Regulation'''- it was found that PGI is regulated only by the relative concentrations of glucose-6-phosphate and fructose 6-phosphate, towards equilibrium. Nevertheless, it was found that the kinetic parameters of PGI does depend on the pH and temperature of the environment. | ||
The following kinetic parameters are proposed for rabbit PGI at pH 8.5 and 30°C <ref>PMID: 5647261</ref> | The following kinetic parameters are proposed for rabbit PGI at pH 8.5 and 30°C <ref>PMID: 5647261</ref> | ||
[[Image:Kinetic_PGI.JPG]] | [[Image:Kinetic_PGI.JPG]] | ||
