Sandbox 160: Difference between revisions

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== Active Site in Detail ==

[[Image:1vc2 image.png | left| thumb |~~Upright~~ = 2.5| Figure 1. Illustration created using PDB software highlighting the NAD+ ligand and Cysteine and Histidine residues within the active site of 1vc2.]] Once Glyceraldehyde 3-phophate comes into contact with the active site it forms a hydrogen bond through its C2 hydroxyl group to Cys149N. The C1 hydroxyl group of the substrate binds to His176NE2. Additional hydrogen bonds to the phosphate group of the substrate from additional residues such as Thr1790G1, Arg231NH1(these two residues are not highlighted in the below illustration) along with N7N and 02'N of the NAD+ moeity (pink) help stabilize the molecule during the course of the reaction in the active site. The nicotinamide ring of the NAD+ ligand is responsible for orienting the hydrogen atom at C1 towards itself which allows for easier transfer in producing in reducing NAD+ to NADH.

[[Image:1vc2 image.png | left|thumb|upright=2 |Figure 1. Illustration created using PDB software highlighting the NAD+ ligand and Cysteine and Histidine residues within the active site of 1vc2.]] Once Glyceraldehyde 3-phophate comes into contact with the active site it forms a hydrogen bond through its C2 hydroxyl group to Cys149N. The C1 hydroxyl group of the substrate binds to His176NE2. Additional hydrogen bonds to the phosphate group of the substrate from additional residues such as Thr1790G1, Arg231NH1(these two residues are not highlighted in the below illustration) along with N7N and 02'N of the NAD+ moeity (pink) help stabilize the molecule during the course of the reaction in the active site. The nicotinamide ring of the NAD+ ligand is responsible for orienting the hydrogen atom at C1 towards itself which allows for easier transfer in producing in reducing NAD+ to NADH.

Sandbox 160: Difference between revisions

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Revision as of 23:39, 31 March 2010 view source Satvir Gill (talk \| contribs) 60 edits No edit summary ← Older edit		Revision as of 00:01, 1 April 2010 view source Satvir Gill (talk \| contribs) 60 edits No edit summary Newer edit →
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	== Active Site in Detail ==		== Active Site in Detail ==
	[[Image:1vc2 image.png \| left\| thumb \|~~Upright~~ = 2.5\| Figure 1. Illustration created using PDB software highlighting the NAD+ ligand and Cysteine and Histidine residues within the active site of 1vc2.]] Once Glyceraldehyde 3-phophate comes into contact with the active site it forms a hydrogen bond through its C2 hydroxyl group to Cys149N. The C1 hydroxyl group of the substrate binds to His176NE2. Additional hydrogen bonds to the phosphate group of the substrate from additional residues such as Thr1790G1, Arg231NH1(these two residues are not highlighted in the below illustration) along with N7N and 02'N of the NAD+ moeity (pink) help stabilize the molecule during the course of the reaction in the active site. The nicotinamide ring of the NAD+ ligand is responsible for orienting the hydrogen atom at C1 towards itself which allows for easier transfer in producing in reducing NAD+ to NADH.		[[Image:1vc2 image.png \| left\|thumb\|upright=2 \|Figure 1. Illustration created using PDB software highlighting the NAD+ ligand and Cysteine and Histidine residues within the active site of 1vc2.]] Once Glyceraldehyde 3-phophate comes into contact with the active site it forms a hydrogen bond through its C2 hydroxyl group to Cys149N. The C1 hydroxyl group of the substrate binds to His176NE2. Additional hydrogen bonds to the phosphate group of the substrate from additional residues such as Thr1790G1, Arg231NH1(these two residues are not highlighted in the below illustration) along with N7N and 02'N of the NAD+ moeity (pink) help stabilize the molecule during the course of the reaction in the active site. The nicotinamide ring of the NAD+ ligand is responsible for orienting the hydrogen atom at C1 towards itself which allows for easier transfer in producing in reducing NAD+ to NADH.