Disulfide Connectivity of Velaglucerase: Difference between revisions
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Human beta-glucocerebrosidase, also known as glucoceremidase, GCase, and velaglucerase, is an enzyme that is commonly found to be deficient in patients with Gaucher's disease. <ref group="xtra">PMID:12792654</ref><ref group="xtra">PMID:17524049</ref> | Human beta-glucocerebrosidase, also known as glucoceremidase, GCase, and velaglucerase, is an enzyme that is commonly found to be deficient in patients with Gaucher's disease. <ref group="xtra">PMID:12792654</ref><ref group="xtra">PMID:17524049</ref> | ||
Currently, there are | Currently, there are three drugs on the market to treat this disease. One being Genzyme's Cerezyme, by Protalix's Taliglucerase and SHIRE's VPRIV. | ||
The typical method used to sequence current biologics, as well as map post translational modifications, is a combination of proteolytic digestion followed by LC/MS. Unfortunately, sometimes this method is not enough for complete identification of some post translational modifications. I am interested in the oxidation of Cys residues for the formation of disulfide bonds. | The typical method used to sequence current biologics, as well as map post translational modifications, is a combination of proteolytic digestion followed by LC/MS. Unfortunately, sometimes this method is not enough for complete identification of some post translational modifications. I am interested in the oxidation of Cys residues for the formation of disulfide bonds. | ||
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'''Disulfide Connectivity:''' ''C4-C16 & C18-C23'' | '''Disulfide Connectivity:''' ''C4-C16 & C18-C23'' | ||
==Reference== | ==Reference== | ||
