2jmh: Difference between revisions
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New page: left|200px<br /> <applet load="2jmh" size="450" color="white" frame="true" align="right" spinBox="true" caption="2jmh" /> '''NMR solution structure of Blo t 5, a major ... |
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[[Image:2jmh. | [[Image:2jmh.jpg|left|200px]]<br /><applet load="2jmh" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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'''NMR solution structure of Blo t 5, a major mite allergen from Blomia tropicalis'''<br /> | '''NMR solution structure of Blo t 5, a major mite allergen from Blomia tropicalis'''<br /> | ||
==Overview== | |||
Blo t 5 is the major allergen from Blomia tropicalis mites and shows, strong IgE reactivity with up to 90% of asthmatic and rhinitis patients', sera. The NMR solution structure of Blo t 5 comprises three long alpha, helices, forming a coiled-coil, triple-helical bundle with a left-handed, twist. TROSY-NMR experiments were used to study Blo t 5 interaction with, the Fab' of a specific monoclonal antibody, mAb 4A7. The mAb epitope, comprises two closely spaced surfaces, I and II, connected by a sharp turn, and bearing critical residues Asn46 and Lys47 on one surface, and Lys54, and Arg57 on the other. This discontinuous epitope overlaps with the human, IgE epitope(s) of Blo t 5. Epitope surface II is further predicted to, undergo conformational exchange in the millisecond to microsecond range., The results presented are critical for the design of a hypoallergenic Blo, t 5 for efficacious immunotherapy of allergic diseases. | |||
==About this Structure== | ==About this Structure== | ||
2JMH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Blomia_tropicalis Blomia tropicalis]. Full crystallographic information is available from [http:// | 2JMH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Blomia_tropicalis Blomia tropicalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JMH OCA]. | ||
==Reference== | |||
Roles of Structure and Structural Dynamics in the Antibody Recognition of the Allergen Proteins: An NMR Study on Blomia tropicalis Major Allergen., Naik MT, Chang CF, Kuo IC, Kung CC, Yi FC, Chua KY, Huang TH, Structure. 2008 Jan;16(1):125-36. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18184590 18184590] | |||
[[Category: Blomia tropicalis]] | [[Category: Blomia tropicalis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: group 5]] | [[Category: group 5]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 10:39:13 2008'' | ||
