RNA Interference: Difference between revisions

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[[RNA Interference]] (RNAi) is a system in living cells that helps control the level of activity of genes. In RNAi, a long double stranded [[RNA]] is cleaved into shorter strands. One strand known as the guide strand is complimentary to the mRNA gene product of a gene of interest. This guide strand is incorporated into an RNA-induced silencing complex (RISC) and subsequently binds the mRNA of the gene of interest. After binding, the RNA/RISC complex subsequently silences the mRNA of interest via cleavage of the mRNA strand.

{{Structure

|PDB= 3dlh |SIZE=400|SCENE=RNA_Interference/3dlh/1|CAPTION= guide-strand-containing Argonaute protein silencing complex [[3dlh]], resolution 3.00Å (<scene name='RNA_Interference/3dlh/1'>initial scene</scene>)

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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dlh OCA], [http://www.ebi.ac.uk/pdbsum/3dlh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3dlh RCSB]</span>

}}

[[RNA Interference]] (RNAi) is a system in living cells that helps control the level of activity of genes. In RNAi, a long double stranded [[RNA]] is cleaved into shorter strands. One strand known as the guide strand is complimentary to the mRNA gene product of a gene of interest. This guide strand is incorporated into an RNA-induced silencing complex (RISC) and subsequently binds the mRNA of the gene of interest. After binding, the RNA/RISC complex subsequently silences the mRNA of interest via cleavage of the mRNA strand.

[[Image:500px-ShRNA Lentivirus.png|350px|right|thumb| Schematic of RNAi Interference in Mammalian Cells]]

{{Structure

|PDB= 1rpu |SIZE=400|SCENE=User:Wayne_Decatur/Plant_Viral_Protein_p19_Suppression_of_RNA_Silencing/View1/2|CAPTION= viral silencing suppressor bound to a siRNA (1rpu), resolution 2.50Å (<scene name='User:Wayne_Decatur/Plant_Viral_Protein_p19_Suppression_of_RNA_Silencing/View1/2'>initial scene</scene>)

|PDB= 1rpu |SIZE=400|ALIGN=Left|SCENE=User:Wayne_Decatur/Plant_Viral_Protein_p19_Suppression_of_RNA_Silencing/View1/2|CAPTION= viral silencing suppressor bound to a siRNA (1rpu), resolution 2.50Å (<scene name='User:Wayne_Decatur/Plant_Viral_Protein_p19_Suppression_of_RNA_Silencing/View1/2'>initial scene</scene>)

|SITE=

|LIGAND=

RNA Interference: Difference between revisions

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@@ Line 1: / Line 1: @@
-[[RNA Interference]] (RNAi) is a system in living cells that helps control the level of activity of genes. In RNAi, a long double stranded [[RNA]] is cleaved into shorter strands. One strand known as the guide strand is complimentary to the mRNA gene product of a gene of interest. This guide strand is incorporated into an RNA-induced silencing complex (RISC) and subsequently binds the mRNA of the gene of interest. After binding, the RNA/RISC complex subsequently silences the mRNA of interest via cleavage of the mRNA strand.
 {{Structure
 |PDB= 3dlh |SIZE=400|SCENE=RNA_Interference/3dlh/1|CAPTION=  guide-strand-containing Argonaute protein silencing complex [[3dlh]], resolution 3.00&Aring; (<scene name='RNA_Interference/3dlh/1'>initial scene</scene>)
@@ Line 10: / Line 9: @@
 |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dlh OCA], [http://www.ebi.ac.uk/pdbsum/3dlh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3dlh RCSB]</span>
 }}
+[[RNA Interference]] (RNAi) is a system in living cells that helps control the level of activity of genes. In RNAi, a long double stranded [[RNA]] is cleaved into shorter strands. One strand known as the guide strand is complimentary to the mRNA gene product of a gene of interest. This guide strand is incorporated into an RNA-induced silencing complex (RISC) and subsequently binds the mRNA of the gene of interest. After binding, the RNA/RISC complex subsequently silences the mRNA of interest via cleavage of the mRNA strand.
 [[Image:500px-ShRNA Lentivirus.png|350px|right|thumb| Schematic of RNAi Interference in Mammalian Cells]]
 {{Structure
-|PDB= 1rpu |SIZE=400|SCENE=User:Wayne_Decatur/Plant_Viral_Protein_p19_Suppression_of_RNA_Silencing/View1/2|CAPTION= viral silencing suppressor bound to a siRNA (1rpu), resolution 2.50&Aring; (<scene name='User:Wayne_Decatur/Plant_Viral_Protein_p19_Suppression_of_RNA_Silencing/View1/2'>initial scene</scene>)
+|PDB= 1rpu |SIZE=400|ALIGN=Left|SCENE=User:Wayne_Decatur/Plant_Viral_Protein_p19_Suppression_of_RNA_Silencing/View1/2|CAPTION= viral silencing suppressor bound to a siRNA (1rpu), resolution 2.50&Aring; (<scene name='User:Wayne_Decatur/Plant_Viral_Protein_p19_Suppression_of_RNA_Silencing/View1/2'>initial scene</scene>)
 |SITE=
 |LIGAND=