2jp8: Difference between revisions

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[[Image:2jp8.gif|left|200px]]<br />

[[Image:2jp8.gif|left|200px]]<br /><applet load="2jp8" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="2jp8" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="2jp8" />

'''Angiotensin 1-7'''<br />

==~~Disease~~==

==Overview==

~~Known diseases associated with this structure: Hypertension~~, ~~essential~~, ~~susceptibility to OMIM:[[http://www~~.~~ncbi~~.~~nlm~~.~~nih.gov/entrez/dispomim.cgi?id=106150 106150]]~~, ~~Preeclampsia~~, ~~susceptibility to OMIM:[[http://www~~.~~ncbi.nlm.nih~~.~~gov~~/~~entrez~~/~~dispomim.cgi?id=106150 106150]]~~, ~~Renal tubular dysgenesis OMIM:[[http://www~~.~~ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=106150 106150]]~~

We report the complete sequence-specific hydrogen NMR assignments of, vasoactive peptide angiotensin-(1-7) (Ang-(1-7)). Assignments of the, majority of the resonances were accomplished by COSY, TOCSY, and ROESY, peak coordinates at 400MHz and 600MHz. Long-side-chain amino acid spin, system identification was facilitated by long-range coherence transfer, experiments (TOCSY). Problems with overlapped resonance signals were, solved by analysis of heteronuclear 2D experiments (HSQC and HMBC)., Nuclear Overhauser effects (NOE) results were used to probe peptide, conformation. We show that the inclusion of the angiotensin-(1-7) tyrosine, residue is favored in inclusion complexes with beta-cyclodextrin. QM/MM, simulations at the DFTB/UFF level confirm the experimental NMR findings, and provide detailed structural information on these compounds in aqueous, solution.

==About this Structure==

2JP8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2JP8 OCA].

2JP8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JP8 OCA].

==Reference==

Study of angiotensin-(1-7) vasoactive peptide and its beta-cyclodextrin inclusion complexes: complete sequence-specific NMR assignments and structural studies., Lula I, Denadai AL, Resende JM, de Sousa FB, de Lima GF, Pilo-Veloso D, Heine T, Duarte HA, Santos RA, Sinisterra RD, Peptides. 2007 Nov;28(11):2199-210. Epub 2007 Aug 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17904691 17904691]

[[Category: Single protein]]

[[Category: Denadai, A.L.]]

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[[Category: signaling protein]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov 12 22~~:57:~~07 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:01:33 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2jp8.gif|left|200px]]<br />
+[[Image:2jp8.gif|left|200px]]<br /><applet load="2jp8" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="2jp8" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="2jp8" />
 '''Angiotensin 1-7'''<br />
-==Disease==
+==Overview==
-Known diseases associated with this structure: Hypertension, essential, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=106150 106150]], Preeclampsia, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=106150 106150]], Renal tubular dysgenesis OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=106150 106150]]
+We report the complete sequence-specific hydrogen NMR assignments of, vasoactive peptide angiotensin-(1-7) (Ang-(1-7)). Assignments of the, majority of the resonances were accomplished by COSY, TOCSY, and ROESY, peak coordinates at 400MHz and 600MHz. Long-side-chain amino acid spin, system identification was facilitated by long-range coherence transfer, experiments (TOCSY). Problems with overlapped resonance signals were, solved by analysis of heteronuclear 2D experiments (HSQC and HMBC)., Nuclear Overhauser effects (NOE) results were used to probe peptide, conformation. We show that the inclusion of the angiotensin-(1-7) tyrosine, residue is favored in inclusion complexes with beta-cyclodextrin. QM/MM, simulations at the DFTB/UFF level confirm the experimental NMR findings, and provide detailed structural information on these compounds in aqueous, solution.
 ==About this Structure==
-JP8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2JP8 OCA].
+JP8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JP8 OCA].
+==Reference==
+Study of angiotensin-(1-7) vasoactive peptide and its beta-cyclodextrin inclusion complexes: complete sequence-specific NMR assignments and structural studies., Lula I, Denadai AL, Resende JM, de Sousa FB, de Lima GF, Pilo-Veloso D, Heine T, Duarte HA, Santos RA, Sinisterra RD, Peptides. 2007 Nov;28(11):2199-210. Epub 2007 Aug 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17904691 17904691]
 [[Category: Single protein]]
 [[Category: Denadai, A.L.]]
@@ Line 23: / Line 25: @@
 [[Category: signaling protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 22:57:07 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:01:33 2008''