Net charge: Difference between revisions
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In a solution of a given pH, a protein will have a negative or positive net charge dependent on the pKa values of the acidic and basic groups of the | In a solution of a given pH, a protein will have a negative or positive net charge dependent on the pKa values of the acidic and basic groups of the constituent amino acids. The [[isoelectric point]], or pI, is the pH at which a protein has zero net charge. When the pH is higher than the isoelectric point, the protein has negative net charge, and when lower, positive net charge. | ||
<br>You can calculate the net charge of your protein easily at a given pH or over a range: | <br>You can calculate the net charge of your protein easily at a given pH or over a range: | ||
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==See Also== | ==See Also== | ||
*[[Isoelectric point]] | *[[Isoelectric point]] | ||
*[[Monovalent Cation Binding in Cubic Insulin Crystals | *[[9ins|Monovalent Cation Binding in Cubic Insulin Crystals]] | ||
==Content Attribution== | ==Content Attribution== | ||
The calculation method was adapted from [http://www.umass.edu/molvis/workshop/osaka09m.htm a class syllabus taught by Eric Martz]. | The calculation method was adapted from [http://www.umass.edu/molvis/workshop/osaka09m.htm a class syllabus taught by Eric Martz]. | ||
