Luciferase: Difference between revisions
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[[Image:3iep.png|left|200px|thumb|Crystal Structure of Luciferase [[3iep]]]] | |||
{{STRUCTURE_3iep| PDB=3iep | SIZE=300| SCENE= |right|CAPTION=Luciferase [[3iep]] }} | |||
[[Luciferase]] (Luc) is an oxidative enzyme used in bioluminescence. It catalyzes the oxidation of the pigment luciferin producing oxyluciferin and light. The images at the left and at the right correspond to one representative Luc, ''i.e.'' the crystal structure of firefly Luciferase ([[3iep]]). | |||
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== 3D Structures of Luciferase == | |||
[[3iep]], [[1lci]] – fLuc – firefly<br /> | |||
[[3ier]] – fLuc+PEG 400<br /> | |||
[[1ba3]] – fLuc+bromoform<br /> | |||
[[3ies]] – fLuc+inhibitor<br /> | |||
[[2d1q]] – LcLuc (mutant)+MgATP – ''Luciola cruciata''<br /> | |||
[[2d1r]] - LcLuc (mutant)+oxyluciferin+AMP<br /> | |||
[[2d1s]], [[2d1t]] - LcLuc (mutant)+intermediate analog<br /> | |||
[[3fgc]] – VhLuc α+β chain+FMN – ''Vibrio harveyi''<br /> | |||
[[1luc]], [[1brl]] - VhLuc α+β chain<br /> | |||
[[1bsl]], [[1xkj]] - VhLuc β chain<br /> | |||
[[2psd]], [[2psh]], [[2psj]], [[2pse]], [[2psf]] – Luc (mutant) – ''Renilla reniformis''<br /> | |||
==Additional information== | |||
[[Image:2d1s jena3dviewer image.jpg|left|220px]] | [[Image:2d1s jena3dviewer image.jpg|left|220px]] | ||
== | <applet load='2d1s' size='450' frame='true' align='right' scene='Luciferase/2d1s/1' caption='Luciferase ([[Luciola cruciata luciferase|2d1s]]) {{Link Toggle FancyCartoonHighQualityView}}.' /> | ||
Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms. | Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms. | ||
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*[[Luciola cruciata luciferase]] (Japanese firefly), complexed with Oxyluciferin & AMP ([[2d1r]]) or with an high-energy analogue ([[2d1s]]) | *[[Luciola cruciata luciferase]] (Japanese firefly), complexed with Oxyluciferin & AMP ([[2d1r]]) or with an high-energy analogue ([[2d1s]]) | ||
*[[3ies]],[[3iep]] and [[3ier]] Firefly luciferase (FLuc) bound to its natural substrate ATP and the inhibitor PTC124<ref>PMID: 20194791</ref> | *[[3ies]],[[3iep]] and [[3ier]] Firefly luciferase (FLuc) bound to its natural substrate ATP and the inhibitor PTC124<ref>PMID: 20194791</ref> | ||
