Prp24: Difference between revisions

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== Introduction ==

Prp24 is a nuclear protein associated with the U6 snRNP and functions in the base pair annealing of U6 snRNA to U4 snRNA of the U4 snRNP.

Prp24 is a nuclear protein associated with the U6 snRNP and functions in the base pair annealing of U6 snRNA to U4 snRNA of the U4 snRNP to form the U4/U6 di-snRNP

=== Pre-mRNA Splicing ===

Pre-mRNA splicing is an essential process in eukaryotes that removes non-coding introns from a pre-mRNA transcript and splices coding exons together before the mRNA is exported from the nucleus for translation into a protein. Splicing requires five snRNPs (U1, U2, U4, U5, U6), several other proteins, and the input of energy from ATP. The U1 and U2 snRNPs assemble individually on the pre-mRNA transcript, while U4 and U6 form a U4/U6 di-snRNP before interacting with U5 to form a U4/U6.U5 tri-snRNP that interacts with U1 and U2 at the pre-mRNA transcript. U4 and U1 then depart, and after conformational changes and base pair formation with the pre-mRNA the remaining snRNPs form the catalytically active spliceosome. Two transesterification reactions then occur; the first reaction is the nucleophillic attack of the a phosphate group at the end of the 5' exon by the 2' hydroxyl of a specific adenosine at the branch point sequence of the intron. This is then followed by the nucleophillic attack of the phosphorous group linking the 3' exon to the intron by the 3' hydroxyl of the 5' exon. These splicing reactions, in addition to the addition of 7-methylguanosine 5' cap and a 3' polyadenosine tail, results in a mature mRNA transcript that can be exported from the nucleus and translated into protein.

== Functional Role ==

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 == Introduction ==
-Prp24 is a nuclear protein associated with the U6 snRNP and functions in the base pair annealing of U6 snRNA to U4 snRNA of the U4 snRNP.
+Prp24 is a nuclear protein associated with the U6 snRNP and functions in the base pair annealing of U6 snRNA to U4 snRNA of the U4 snRNP to form the U4/U6 di-snRNP
+=== Pre-mRNA Splicing ===
+Pre-mRNA splicing is an essential process in eukaryotes that removes non-coding introns from a pre-mRNA transcript and splices coding exons together before the mRNA is exported from the nucleus for translation into a protein.  Splicing requires five snRNPs (U1, U2, U4, U5, U6), several other proteins, and the input of energy from ATP.  The U1 and U2 snRNPs assemble individually on the pre-mRNA transcript, while U4 and U6 form a U4/U6 di-snRNP before interacting with U5 to form a U4/U6.U5 tri-snRNP that interacts with U1 and U2 at the pre-mRNA transcript.  U4 and U1 then depart, and after conformational changes and base pair formation with the pre-mRNA the remaining snRNPs form the catalytically active spliceosome.  Two transesterification reactions then occur; the first reaction is the nucleophillic attack of the a phosphate group at the end of the 5' exon by the 2' hydroxyl of a specific adenosine at the branch point sequence of the intron.  This is then followed by the nucleophillic attack of the phosphorous group linking the 3' exon to the intron by the 3' hydroxyl of the 5' exon.  These splicing reactions, in addition to the addition of 7-methylguanosine 5' cap and  a 3' polyadenosine tail, results in a mature mRNA transcript that can be exported from the nucleus and translated into protein.
 == Functional Role ==