Reverse transcriptase: Difference between revisions

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{{STRUCTURE_1hmv|  PDB=1hmv  | SIZE=300| SCENE=Reverse_transcriptase/Cv/1 |right|CAPTION=HIV-1 Reverse transcriptase, [[1hmv]] }}
{{STRUCTURE_1hmv|  PDB=1hmv  | SIZE=300| SCENE=Reverse_transcriptase/Cv/1 |right|CAPTION=HIV-1 Reverse transcriptase, [[1hmv]] }}


[[Reverse transcriptase]] (RT) or RNA-dependent DNA polymerase transcribes single-stranded RNA into double-stranded [[DNA]].  HIV-1 RT is from the human immunodeficiency virus and is a heterodimer of P66 and P51. The images at the left and at the right correspond to one representative RT structure, ''i.e.'' crystal structure of HIV-1 Reverse transcriptase ([[1hmv]]). P15 is its RNAse H domain.  NNRTI are the non-nucleoside inhibitors of HIV-1 RT.  M-MLV is RT from the leukemia virus. Being the protein that gives their name to Retroviruses, Reverse Transcriptase is, in company of [[Hiv protease|Protease]] and [[Hiv integrase|Integrase]], the most important part of the protein system involved in the process of infection of viruses like HIV, MuLV and AMV, and has the unusual property of transcribing ssRNA into dsDNA going against the Central Dogma of Molecular Biology.
[[Reverse transcriptase]] (RT) or RNA-dependent DNA polymerase transcribes single-stranded RNA into double-stranded [[DNA]].  HIV-1 RT is from the human immunodeficiency virus and is a heterodimer of P66 and P51. The images at the left and at the right correspond to one representative RT structure, ''i.e.'' crystal structure of HIV-1 Reverse transcriptase ([[1hmv]]). P15 is its RNAse H domain.  NNRTI are the non-nucleoside inhibitors of HIV-1 RT and NRTI are the nucleoide inhibitors.  M-MLV is RT from the leukemia virus. Being the protein that gives their name to Retroviruses, Reverse Transcriptase is, in company of [[Hiv protease|Protease]] and Integrase, the most important part of the protein system involved in the process of infection of viruses like HIV, MuLV and AMV, and has the unusual property of transcribing ssRNA into dsDNA going against the Central Dogma of Molecular Biology.
Since its discovery in 1970, the study of its properties and mechanisms of action have been of high interest among the scientific community due to the unique properties that makes it an important medical target enzyme and important tool for genetic engineering applications like RT-PCR in the construction of cDNA libraries.  
Since its discovery in 1970, the study of its properties and mechanisms of action have been of high interest among the scientific community due to the unique properties that makes it an important medical target enzyme and important tool for genetic engineering applications like RT-PCR in the construction of cDNA libraries.  


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[[3irx]], [[3is9]], [[3lak]], [[3lal]], [[3lam]], [[3lan]], [[3ffi]], [[3di6]], [[3dle]], [[3dlg]], [[2rki]], [[3c6t]], [[3c6u]], [[2rf2]], [[2vg5]], [[2vg6]], [[2vg7]], [[2opp]], [[1jlq]], [[1ep4]], [[1c0t]], [[1c0u]], [[1dtt]], [[1dtq]], [[1bqm]], [[1rt1]], [[1rt2]], [[1rev]], [[1rtj]], [[1vru]], [[1hnv]], [[1hni]] - P66/P51+NNRTI<br />
[[3irx]], [[3is9]], [[3lak]], [[3lal]], [[3lam]], [[3lan]], [[3ffi]], [[3di6]], [[3dle]], [[3dlg]], [[2rki]], [[3c6t]], [[3c6u]], [[2rf2]], [[2vg5]], [[2vg6]], [[2vg7]], [[2opp]], [[1jlq]], [[1ep4]], [[1c0t]], [[1c0u]], [[1dtt]], [[1dtq]], [[1bqm]], [[1rt1]], [[1rt2]], [[1rev]], [[1rtj]], [[1vru]], [[1hnv]], [[1hni]] - P66/P51+NNRTI<br />
[[1s1t]], [[1s1v]], [[1s1w]], [[1s6p]], [[1s6q]], [[1s9e]], [[1s9g]], [[1suq]], [[1sv5]], [[3dm2]], [[3dmj]], [[3dok]], [[3dol]], [[2opq]], [[2opr]], [[2ops]], [[2ic3]], [[2hnz]], [[1jlg]], [[1bqn]], [[1uwb]], [[1tvr]]  - P66/P51 (mutant)+NNRTI<br />
[[1s1t]], [[1s1v]], [[1s1w]], [[1s6p]], [[1s6q]], [[1s9e]], [[1s9g]], [[1suq]], [[1sv5]], [[3dm2]], [[3dmj]], [[3dok]], [[3dol]], [[2opq]], [[2opr]], [[2ops]], [[2ic3]], [[2hnz]], [[1jlg]], [[1bqn]], [[1uwb]], [[1tvr]]  - P66/P51 (mutant)+NNRTI<br />
[[3kk1]], [[1c1b]], [[1rti]] - P66/P51+nucleotide inhibitor<br />
[[3kk1]], [[1c1b]], [[1rti]] - P66/P51+RTI<br />
[[1jla]] - P66/P51 (mutant)+ nucleotide inhibitor<br />
[[1jla]] - P66/P51 (mutant)+ NRTI<br />
[[3ig1]] - P66/P51+ inhibitor beta-thujaplicinol<br />
[[3ig1]] - P66/P51+ inhibitor beta-thujaplicinol<br />
[[3i0r]], [[3i0s]], [[3m8q]], [[3isn]], [[3ith]], [[1c1c]]  - P66/P51+ pyrimidine derivative<br />
[[3i0r]], [[3i0s]], [[3m8q]], [[3isn]], [[3ith]], [[1c1c]]  - P66/P51+ pyrimidine derivative<br />
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[[1jlc]], [[1iky]], [[1eet]] - P66/P51(mutant)+ pyridine derivative<br />
[[1jlc]], [[1iky]], [[1eet]] - P66/P51(mutant)+ pyridine derivative<br />
[[2b5j]], [[2ban]], [[2be2]], [[1rth]], [[1hvt]] - P66/P51+ pyridone derivative<br />
[[2b5j]], [[2ban]], [[2be2]], [[1rth]], [[1hvt]] - P66/P51+ pyridone derivative<br />
[[1lw2]], [[1rt3]] - P66/P51 (mutant)+ pyridone derivative<br />
[[1lw2]], [[1rt3]] - P66/P51 (mutant)+ NRTI<br />
[[1tkt]], [[1tkx]], [[1tkz]], [[1tl1]], [[1tl3]] - P66/P51+ quinoline derivative<br />
[[1tkt]], [[1tkx]], [[1tkz]], [[1tl1]], [[1tl3]] - P66/P51+ quinoline derivative<br />
[[2b6a]] - P66/P51+ THR-50<br />  
[[2b6a]] - P66/P51+ THR-50<br />  
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[[1j5o]], [[1rtd]] - P66/P51 (mutant)+DNA<br />
[[1j5o]], [[1rtd]] - P66/P51 (mutant)+DNA<br />
[[1hvu]] - P66/P51+RNA<br />  
[[1hvu]] - P66/P51+RNA<br />  
[[1t03]], [[1t05]] - P66/P51+DNA+Fab+tenofovir<br />
[[1t03]], [[1t05]] - P66/P51+DNA+Fab+NRTI<br />
[[3jsm]] - P66/P51 (mutant)+DNA+tenofovir<br />
[[3jsm]] - P66/P51 (mutant)+DNA+NRTI<br />
[[2hmi]] - P66/P51 (mutant)+DNA+Fab<br />
[[2hmi]] - P66/P51 (mutant)+DNA+Fab<br />
[[2iaj]] - P66/P51 (mutant)+ATP<br />
[[2iaj]] - P66/P51 (mutant)+NRTI<br />
[[3kk2]] - P66/P51+DNA+ATP<br />
[[3kk2]] - P66/P51+DNA+NRTI<br />
[[3jyt]] - P66/P51 (mutant)+DNA+ATP<br />
[[3jyt]] - P66/P51 (mutant)+DNA+NRTI<br />


===HIV-1 RT P66===
===HIV-1 RT P66===
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[[1o1w]] – P15+Mg - NMR<br />
[[1o1w]] – P15+Mg - NMR<br />


===HIV-2 RT===
[[1mu2]] - RT (mutant)


=== Leukemia virus RT ===
=== Leukemia virus RT ===
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[[2hb5]], [[1mml]] - catalytic fragment<br />
[[2hb5]], [[1mml]] - catalytic fragment<br />
[[2r2r]], [[2r2t]], [[2fjv]], [[2fjw]] – catalytic fragment+DNA<br />
[[2r2r]], [[2r2t]], [[2fjv]], [[2fjw]], [[2fjx]], [[1ztw]], [[2fvs]], [[2fvr]], [[2fvq]], [[2fvp]], [[1n4l]] – catalytic fragment+DNA<br />
[[2r2s]], [[2r2u]] - catalytic fragment+DNA+Co Bleomycin<br />
[[2r2s]], [[2r2u]] - catalytic fragment+DNA+Co Bleomycin<br />
[[2fjx]], [[1ztw]] - catalytic fragment+DNA<br />
[[1ztt]] - catalytic fragment+DNA+netropsin<br />
[[1ztt]] - catalytic fragment+DNA+netropsin<br />
[[1rw3]], [[1nnd]] – fragment (mutant)<br />
[[1rw3]], [[1nnd]] – fragment (mutant)<br />
[[1i6j]], [[1d0e]], [[1d1u]], [[1qaj]], [[1qai]] – N-terminal+DNA<br />
[[1i6j]], [[1d0e]], [[1d1u]], [[1qaj]], [[1qai]] – N-terminal+DNA<br />
[[3fsi]] - catalytic fragment+DNA+trypanocidal
===RT from telomerase===
[[2b2a]] - RT telomerase catalytic subunit (mutant) - ''Tetrahymena thermophila''<br />
[[2r4g]] - RT telomerase RNA-binding domain<br />
[[3du6]], [[3du5]] - TcRT telomerase catalytic subunit - ''Tribolium castaneum''<br />
[[3kyl]] - TcRT telomerase catalytic subunit + DNA


==See Also==
==See Also==
Retrieved from "https://proteopedia.org/Reverse_transcriptase"