Sandbox 51: Difference between revisions
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As seen above, lysozyme works by hydrolyzing the glycosidic bond, distorting the bond between the N-acetylglucosamine sugar and the N-acetylmuramic acid sugar. This produces a glycosyl enzyme intermediate, which reacts with a water molecule to produce the product and the unchanged enzyme. | As seen above, lysozyme works by hydrolyzing the glycosidic bond, distorting the bond between the N-acetylglucosamine sugar and the N-acetylmuramic acid sugar. This produces a glycosyl enzyme intermediate, which reacts with a water molecule to produce the product and the unchanged enzyme. | ||
=== Inhibitors === | |||
Lysozyme is best inhibited by small saccharides which act competitively with the natural substrate. The smaller saccharides will bind to the first three binding sites of the cleft (sites A-C), but not reach sites D and E, where the enzyme cuts the glycosidic bond. So, the competitive inhibitor will stick in the cleft, not allowing the substrate to bind to the enzyme complex.<ref>http://mcdb-webarchive.mcdb.ucsb.edu/sears/biochemistry/tw-enz/lysozyme/HEWL/lysozyme-overview.htm</ref> Several known inhibitors of lysozyme are SDS, N-acetyl-D-glucosamine, and various alcohols and oxidizing agents.<ref>http://www.worthington-biochem.com/ly/default.html</ref> | |||
=== Zymogen of Lysozyme: Enzymatic Precursor === | === Zymogen of Lysozyme: Enzymatic Precursor === | ||
