Prp24: Difference between revisions

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=== Lsm Proteins ===

The Lsm proteins are a set of seven proteins found in the U6 snRNP that form a ring around the 3' end of U6 (reference _____). An interaction between Prp24 and the Lsm proteins was suggested upon identification of the Lsms and the fact that formation of U4/U6 by Prp24 was less efficient in deproteinized solutions of U4 and U6 snRNAs (reference Raghunathan and Guthrie), as well as the identification of a genetic interaction between the genes for Prp24 and Lsm4 (Mayes et al. 1999). This proposed interaction was further supported by UV cross-linking experiments in which both Prp24 and Lsm4 were immunoprecipitated with U6 after cross-linking (reference Vidal et al. 1999). It has also been shown that both Prp24 and Lsm4, as well as the other Lsm proteins, require the 3' end of U6 in order to interact with the snRNA (Vidal et al. 1999), suggesting that this may be the region where the proteins interact.

The Lsm proteins are a set of seven proteins found in the U6 snRNP that form a ring around the 3' end of U6 (reference _____). An interaction between Prp24 and the Lsm proteins was suggested upon identification of the Lsms and the fact that formation of U4/U6 by Prp24 was less efficient in deproteinized solutions of U4 and U6 snRNAs (reference Raghunathan and Guthrie), as well as the identification of a genetic interaction between the genes for Prp24 and Lsm4 (Mayes et al. 1999). This proposed interaction was further supported by UV cross-linking experiments in which both Prp24 and Lsm4 were immunoprecipitated with U6 after cross-linking (reference Vidal et al. 1999). It has also been shown that both Prp24 and Lsm4, as well as the other Lsm proteins, require the 3' end of U6 in order to interact with the snRNA (Vidal et al. 1999), suggesting that this may be the region where the proteins interact. Genome-wide protein interaction screens showed that Prp24 interacts additionally with Lsm 2, 5, 6, 7 and 8, and that overexpression of either Prp24 or Lsm4 can complement or intensify the effects of a defect in the other protein (Fromont-Racine et al. 2000), further supporting an interaction between the Lsm proteins and Prp24.

===Additional Interactions===

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@@ Line 60: / Line 60: @@
 === Lsm Proteins ===
-The Lsm proteins are a set of seven proteins found in the U6 snRNP that form a ring around the 3' end of U6 (reference _____).  An interaction between Prp24 and the Lsm proteins was suggested upon identification of the Lsms and the fact that formation of U4/U6 by Prp24 was less efficient in deproteinized solutions of U4 and U6 snRNAs (reference Raghunathan and Guthrie), as well as the identification of a genetic interaction between the genes for Prp24 and Lsm4 (Mayes et al. 1999).  This proposed interaction was further supported by UV cross-linking experiments in which both Prp24 and Lsm4 were immunoprecipitated with U6 after cross-linking (reference Vidal et al. 1999).  It has also been shown that both Prp24 and Lsm4, as well as the other Lsm proteins, require the 3' end of U6 in order to interact with the snRNA (Vidal et al. 1999), suggesting that this may be the region where the proteins interact.
+The Lsm proteins are a set of seven proteins found in the U6 snRNP that form a ring around the 3' end of U6 (reference _____).  An interaction between Prp24 and the Lsm proteins was suggested upon identification of the Lsms and the fact that formation of U4/U6 by Prp24 was less efficient in deproteinized solutions of U4 and U6 snRNAs (reference Raghunathan and Guthrie), as well as the identification of a genetic interaction between the genes for Prp24 and Lsm4 (Mayes et al. 1999).  This proposed interaction was further supported by UV cross-linking experiments in which both Prp24 and Lsm4 were immunoprecipitated with U6 after cross-linking (reference Vidal et al. 1999).  It has also been shown that both Prp24 and Lsm4, as well as the other Lsm proteins, require the 3' end of U6 in order to interact with the snRNA (Vidal et al. 1999), suggesting that this may be the region where the proteins interact.  Genome-wide protein interaction screens showed that Prp24 interacts additionally with Lsm 2, 5, 6, 7 and 8, and that overexpression of either Prp24 or Lsm4 can complement or intensify the effects of a defect in the other protein (Fromont-Racine et al. 2000), further supporting an interaction between the Lsm proteins and Prp24.
 ===Additional Interactions===