Prp24: Difference between revisions

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U6 is considered to be one of the most catalytically important snRNAs in the spliceosome, as it interacts directly with the 5' splice site through base pairing <ref name="Fortner">PMID:8299941</ref>. It is thought to undergoe three conformational changes throughout the entire process of splicing and splicesome assembly; it exists as one conformation as free U6 snRNP, another conformation as part of the U4/U6 di-snRNP and a third conformation associated with U2 and the pre-mRNA <ref name="Jandrositz">PMID:7882985</ref>. In addition to Prp24, U6 is associated with seven other proteins, Lsm 2-8, which form a ring around the 3' portion of the U6 snRNA <ref name="Karaduman2008">PMID:18971323</ref>.

The U4 snRNA is though to be non-catalytic because it leaves the spliceosome before the transesterification reactions occur. Its function is instead thought to be aiding U6 in maintaining a conformation that will enable it to interact with U2 and the 5' splice site. The U4 snRNP contains the U4 snRNA, a ring complex of the Sm proteins B-G, and the proteins Prp3, Prp4, and Snu13 ~~(reference~~ Brow ~~2002)~~.

The U4 snRNA is though to be non-catalytic because it leaves the spliceosome before the transesterification reactions occur <ref name="Staley"/>. Its function is instead thought to be aiding U6 in maintaining a conformation that will enable it to interact with U2 and the 5' splice site. The U4 snRNP contains the U4 snRNA, a ring complex of the Sm proteins B-G, and the proteins Prp3, Prp4, and Snu13 <ref name="Brow review"/>.

The annealing of the U4 and U6 snRNAs to form the U4/U6 di-snRNP complex, which contains the additional proteins Prp6 and Prp31, is an essential process in the formation of the spliceosome. It enables interaction with the U5 snRNP to form the U4/U6.U5 complex which serves to deliver the U5 and U6 snRNPs to the appropriate sites in the pre-spliceosome complex to form a catalytically active spliceosome upon departure of U1 and U4.

The annealing of the U4 and U6 snRNAs to form the U4/U6 di-snRNP complex, which contains the additional proteins Prp6 and Prp31, is an essential process in the formation of the spliceosome <ref name="Brow review"/>. It enables interaction with the U5 snRNP to form the U4/U6.U5 complex which serves to deliver the U5 and U6 snRNPs to the appropriate sites in the pre-spliceosome complex to form a catalytically active spliceosome upon departure of U1 and U4 <ref name="Brow/>.

===Role of Prp24 in Splicing===

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 U6 is considered to be one of the most catalytically important snRNAs in the spliceosome, as it interacts directly with the 5' splice site through base pairing <ref name="Fortner">PMID:8299941</ref>.  It is thought to undergoe three conformational changes throughout the entire process of splicing and splicesome assembly; it exists as one conformation as free U6 snRNP, another conformation as part of the U4/U6 di-snRNP and a third conformation associated with U2 and the pre-mRNA <ref name="Jandrositz">PMID:7882985</ref>.  In addition to Prp24, U6 is associated with seven other proteins, Lsm 2-8, which form a ring around the 3' portion of the U6 snRNA <ref name="Karaduman2008">PMID:18971323</ref>.
-The U4 snRNA is though to be non-catalytic because it leaves the spliceosome before the transesterification reactions occur.  Its function is instead thought to be aiding U6 in maintaining a conformation that will enable it to interact with U2 and the 5' splice site.  The U4 snRNP contains the U4 snRNA, a ring complex of the Sm proteins B-G, and the proteins Prp3, Prp4, and Snu13 (reference Brow 2002).
+The U4 snRNA is though to be non-catalytic because it leaves the spliceosome before the transesterification reactions occur <ref name="Staley"/>.  Its function is instead thought to be aiding U6 in maintaining a conformation that will enable it to interact with U2 and the 5' splice site.  The U4 snRNP contains the U4 snRNA, a ring complex of the Sm proteins B-G, and the proteins Prp3, Prp4, and Snu13 <ref name="Brow review"/>.
-The annealing of the U4 and U6 snRNAs to form the U4/U6 di-snRNP complex, which contains the additional proteins Prp6 and Prp31, is an essential process in the formation of the spliceosome.  It enables interaction with the U5 snRNP to form the U4/U6.U5 complex which serves to deliver the U5 and U6 snRNPs to the appropriate sites in the pre-spliceosome complex to form a catalytically active spliceosome upon departure of U1 and U4.
+The annealing of the U4 and U6 snRNAs to form the U4/U6 di-snRNP complex, which contains the additional proteins Prp6 and Prp31, is an essential process in the formation of the spliceosome <ref name="Brow review"/>.  It enables interaction with the U5 snRNP to form the U4/U6.U5 complex which serves to deliver the U5 and U6 snRNPs to the appropriate sites in the pre-spliceosome complex to form a catalytically active spliceosome upon departure of U1 and U4 <ref name="Brow/>.
 ===Role of Prp24 in Splicing===