Sandbox Reserved 198: Difference between revisions
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The RNase 1-118 was prepared by successive digestion of RNase A pepsin and carboxypeptidase A (Doscher, 1983). The synthetic component, RNase 111-124, was prepared by the use of solid-phase peptide synthetic mothods, in which the peptide chain was assembled in the stepwise mannar while it was attached at one end to a solid support. The peptide chain was extented by repetitive steps of deprotection, neutralization and coupling until the desired sequence was obtained (Lin, 1970). It was important that the synthesis proceeds rapidly and in high yields to prevent side reactions or by-products. | The RNase 1-118 was prepared by successive digestion of RNase A pepsin and carboxypeptidase A (Doscher, 1983). The synthetic component, RNase 111-124, was prepared by the use of solid-phase peptide synthetic mothods, in which the peptide chain was assembled in the stepwise mannar while it was attached at one end to a solid support. The peptide chain was extented by repetitive steps of deprotection, neutralization and coupling until the desired sequence was obtained (Lin, 1970). It was important that the synthesis proceeds rapidly and in high yields to prevent side reactions or by-products. | ||
'''Related Web-links''' | |||
1. http://en.wikipedia.org/wiki/Ribonuclease_A | |||
2. http://www.uta.edu/faculty/sawasthi/Enzymology-4351-5324/Class%20Syllabus%20Enzymology/ribonucleaseA.pdf | |||
3. http://chemical-quantum-images.blogspot.com/2008/11/ribonuclease.html | |||
4. http://www.ffame.org/sbenner/compbiochemphysc123.103-111.pdf | |||
'''References''' | '''References''' | ||
