Sandbox Reserved 325: Difference between revisions
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here is a <scene name='Sandbox_Reserved_325/Disulfide/1'>disulfide bridge</scene> | here is a <scene name='Sandbox_Reserved_325/Disulfide/1'>disulfide bridge</scene> | ||
==Mechanism== | ==Mechanism== | ||
in michaelis menten kinetics it has Km of 0.5 ± 0.05 mM and Kcat of 60 s<sup>-1<sup | in michaelis menten kinetics it has Km of 0.5 ± 0.05 mM and Kcat of 60 s<sup>-1 </sup> | ||
Chorismate mutase is an essential enzyme in the shikimate pathway <ref name="pizza"> PMID:17146044 </ref>. This pathway allows for the biosynthesis of aromatic amino acids tryptophan, tyrosine, and phenylalanine <ref name="pizza" />. The production of tyrosine and phenylalanine is achieved by what is called a Claisen arrangement. first converting chorismate to prephenate. Prephenate then reacts with prephenate dehydratase and prephenate dehydrogenase which forms phenylpyruvate and hydroxyphenylpyruvate. After this occursm aminotransferase converts hydroxy-phenylpyruvate and phenylpyruvate to phenylalanine and tyrosine. Chorismate mutase provides a 2x10<sup>6</sup> fold increase in the rate of reaction, in comparison to the uncatalyzed reaction <ref > P.D. Lyne, A.J. Mulholland, W.G. Richards. Insights into chorismate mutase catalysis from a combined qm/mm simulation of the enzyme reaction. Journal of the American Chemistry Society. 1995 117(45):11345-11350</ref>. It is the only example of an enzyme catalyzing a percyclic reaction <ref name="strat"> PMID:10960481 </ref> | Chorismate mutase is an essential enzyme in the shikimate pathway <ref name="pizza"> PMID:17146044 </ref>. This pathway allows for the biosynthesis of aromatic amino acids tryptophan, tyrosine, and phenylalanine <ref name="pizza" />. The production of tyrosine and phenylalanine is achieved by what is called a Claisen arrangement. first converting chorismate to prephenate. Prephenate then reacts with prephenate dehydratase and prephenate dehydrogenase which forms phenylpyruvate and hydroxyphenylpyruvate. After this occursm aminotransferase converts hydroxy-phenylpyruvate and phenylpyruvate to phenylalanine and tyrosine. Chorismate mutase provides a 2x10<sup>6</sup> fold increase in the rate of reaction, in comparison to the uncatalyzed reaction <ref > P.D. Lyne, A.J. Mulholland, W.G. Richards. Insights into chorismate mutase catalysis from a combined qm/mm simulation of the enzyme reaction. Journal of the American Chemistry Society. 1995 117(45):11345-11350</ref>. It is the only example of an enzyme catalyzing a percyclic reaction <ref name="strat"> PMID:10960481 </ref> | ||
