Factor Xa: Difference between revisions

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===Catalytic Triad===

Serine proteases use a ~~Ser-His-Asp~~ [http://en.wikipedia.org/wiki/Catalytic_triad catalytic triad], each playing an important role. The serine donates an OH group to act as a nucleophile and attack the carbonyl group of the peptide bond that will be broken within the substrate. Histidine coordinates the attack of the peptide bond by accepting the hydrogen from the serine –OH group with a pair of electrons on nitrogen. Aspartate contains a carboxyl group to hydrogen bond with the histidine, in order to properly position His, as well as to help with stabilization.

Serine proteases use a His57, Asp102, Ser195 [http://en.wikipedia.org/wiki/Catalytic_triad catalytic triad], each playing an important role. The serine donates an OH group to act as a nucleophile and attack the carbonyl group of the peptide bond that will be broken within the substrate. Histidine coordinates the attack of the peptide bond by accepting the hydrogen from the serine –OH group with a pair of electrons on nitrogen. Aspartate contains a carboxyl group to hydrogen bond with the histidine, in order to properly position His, as well as to help with stabilization.

===Substrate Recognition Sites ===

Factor Xa: Difference between revisions

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 ===Catalytic Triad===
 <applet load='2PR3' size='300' frame='true' align='right' caption='Structure of factor Xa PBD id: 2PR3' />
-Serine proteases use a Ser-His-Asp [http://en.wikipedia.org/wiki/Catalytic_triad catalytic triad], each playing an important role. The serine donates an OH group to act as a nucleophile and attack the carbonyl group of the peptide bond that will be broken within the substrate. Histidine coordinates the attack of the peptide bond by accepting the hydrogen from the serine –OH group with a pair of electrons on nitrogen. Aspartate contains a carboxyl group to hydrogen bond with the histidine, in order to properly position His, as well as to help with stabilization.
+Serine proteases use a His57, Asp102, Ser195 [http://en.wikipedia.org/wiki/Catalytic_triad catalytic triad], each playing an important role. The serine donates an OH group to act as a nucleophile and attack the carbonyl group of the peptide bond that will be broken within the substrate. Histidine coordinates the attack of the peptide bond by accepting the hydrogen from the serine –OH group with a pair of electrons on nitrogen. Aspartate contains a carboxyl group to hydrogen bond with the histidine, in order to properly position His, as well as to help with stabilization.
 ===Substrate Recognition Sites ===