Sandbox Reserved 325: Difference between revisions
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=Chorismate Mutase= | =Chorismate Mutase= | ||
==Introduction== | ==Introduction== | ||
The gene Rv1885c from ''Mycobacteriam tuberculosis'' encodes for a non-functional chorismate mutase (*MtCM)<ref name="pizza" />. This non-functional mutase has a 33-amino-acid cleavable sequence. It is a vital enzyme in the shikimate pathway, which allows for the synthesis of tryptophan, tyrosinem and phenylalanine <ref name="pizza" />. Chorismate mutase only occurs in bacteria, higher plants, and fungi, due to the fact that the shikimate pathway is only found in these organisms <ref name="strat" />. In ''Escherichia coli'', chorismate mutase has a periplasmic destination<ref name="pizza" />. In ''M. tuberculosis'' there is in abscence of a periplasmic compartment for chorismate mutase, so it secretes into the culture filtrate of ''M. tuberculosis''<ref name="pizza" /> | The gene Rv1885c from ''Mycobacteriam tuberculosis'' encodes for a non-functional chorismate mutase (*MtCM)<ref name="pizza" />. This non-functional mutase has a 33-amino-acid cleavable sequence. It is a vital enzyme in the shikimate pathway, which allows for the synthesis of tryptophan, tyrosinem and phenylalanine <ref name="pizza" />. Chorismate mutase only occurs in bacteria, higher plants, and fungi, due to the fact that the shikimate pathway is only found in these organisms <ref name="strat" />. In ''Escherichia coli'', chorismate mutase has a periplasmic destination<ref name="pizza" />. In ''M. tuberculosis'' there is in abscence of a periplasmic compartment for chorismate mutase, so it secretes into the culture filtrate of ''M. tuberculosis''<ref name="pizza" />. It is believed that a pseudoperiplasmic space might exist in ''M. tuberculosis''<ref name="pizza" /> | ||
Rv1185c is synthesized along with an amino acid terminal sequence, which is cleaved off from the mature protein when expressed with e coli | Rv1185c is synthesized along with an amino acid terminal sequence, which is cleaved off from the mature protein when expressed with e coli | ||
