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==TreX==

TreX is an archaeal GDE from the species, [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae~~ ''Sulfolobus ~~solfataricus~~''] <ref name="Woo" />. Interestingly, TreX exhibits 74% sequence similarity to the isoamylase from ''Sulfolobus acidocaldarium'', yet TreX itself reveals both α-1,6-glycosidase and α-1,4-transferase activity. It functions to debranch the side chains of glycogen into maltodextrin, and subsequently TreY and TreZ convert the maltodextrin into trehalose <ref name="Woo" /> <ref name="oligomer"/>. Although TreX exhibits bifunctional activity, its catalytic region differs greatly from other glycogen debranching enzymes. For example, mammalian and yeast GDEs have distinct catalytic sites for the α-1,6-glycosidase activity and α-1,4-transferase activity. These sites are even located at different regions of the polypeptide. In TreX, however, both enzymatic rections take place within the same catalytic region <ref name="Woo" />.

TreX is an archaeal GDE from the species, [http://en.wikipedia.org/wiki/Sulfolobus ''Sulfolobus''] solfataricus <ref name="Woo" />. Interestingly, TreX exhibits 74% sequence similarity to the isoamylase from ''Sulfolobus acidocaldarium'', yet TreX itself reveals both α-1,6-glycosidase and α-1,4-transferase activity. It functions to debranch the side chains of glycogen into maltodextrin, and subsequently TreY and TreZ convert the maltodextrin into trehalose <ref name="Woo" /> <ref name="oligomer"/>. Although TreX exhibits bifunctional activity, its catalytic region differs greatly from other glycogen debranching enzymes. For example, mammalian and yeast GDEs have distinct catalytic sites for the α-1,6-glycosidase activity and α-1,4-transferase activity. These sites are even located at different regions of the polypeptide. In TreX, however, both enzymatic rections take place within the same catalytic region <ref name="Woo" />.

===Structure and Function===

Sandbox Reserved 338: Difference between revisions

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 ==TreX==
-TreX is an archaeal GDE from the species, [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae ''Sulfolobus solfataricus''] <ref name="Woo" />. Interestingly, TreX exhibits 74% sequence similarity to the isoamylase from ''Sulfolobus acidocaldarium'', yet TreX itself reveals both α-1,6-glycosidase and α-1,4-transferase activity. It functions to debranch the side chains of glycogen into maltodextrin, and subsequently TreY and TreZ convert the maltodextrin into trehalose <ref name="Woo" /> <ref name="oligomer"/>. Although TreX exhibits bifunctional activity, its catalytic region differs greatly from other glycogen debranching enzymes. For example, mammalian and yeast GDEs have distinct catalytic sites for the α-1,6-glycosidase activity and α-1,4-transferase activity. These sites are even located at different regions of the polypeptide. In TreX, however, both enzymatic rections take place within the same catalytic region <ref name="Woo" />.
+TreX is an archaeal GDE from the species, [http://en.wikipedia.org/wiki/Sulfolobus ''Sulfolobus''] solfataricus <ref name="Woo" />. Interestingly, TreX exhibits 74% sequence similarity to the isoamylase from ''Sulfolobus acidocaldarium'', yet TreX itself reveals both α-1,6-glycosidase and α-1,4-transferase activity. It functions to debranch the side chains of glycogen into maltodextrin, and subsequently TreY and TreZ convert the maltodextrin into trehalose <ref name="Woo" /> <ref name="oligomer"/>. Although TreX exhibits bifunctional activity, its catalytic region differs greatly from other glycogen debranching enzymes. For example, mammalian and yeast GDEs have distinct catalytic sites for the α-1,6-glycosidase activity and α-1,4-transferase activity. These sites are even located at different regions of the polypeptide. In TreX, however, both enzymatic rections take place within the same catalytic region <ref name="Woo" />.
 ===Structure and Function===