Sandbox reserved 330: Difference between revisions
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The YcdE protein has similar structures to other proteins, such as Pem/Kid from E.coli in plasmid R1, and CcdB from E.coli in plasmid F. These similarities include a five stranded antiparallel sheet and a smaller three stranded β-sheet with a C-terminal α helix. YdcE shares 27% sequence similarity with Pem/Kid and 7% with CcdB. However, the electronegative surface potential of YdcE is more negative than Kid and CcdB, with a pI of 4.7. This is largely due to having six charged amino acids; Asp 96, Asp 97, Glu 98, Glu 105, Asp 101, and Asp 104. | The YcdE protein has similar structures to other proteins, such as Pem/Kid from E.coli in plasmid R1, and CcdB from E.coli in plasmid F. These similarities include a five stranded antiparallel sheet and a smaller three stranded β-sheet with a C-terminal α helix. YdcE shares 27% sequence similarity with Pem/Kid and 7% with CcdB. However, the electronegative surface potential of YdcE is more negative than Kid and CcdB, with a pI of 4.7. This is largely due to having six charged amino acids; Asp 96, Asp 97, Glu 98, Glu 105, Asp 101, and Asp 104. | ||
[[Image:6_charged_AA.jpg | thumb | right | | [[Image:6_charged_AA.jpg | thumb | right | 400px |6 charged amino acids contributing to electronegative surface potential]] | ||
==Active Site== | ==Active Site== | ||
