Sandbox Reserved 325: Difference between revisions
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=Chorismate Mutase= | =Chorismate Mutase= | ||
==Introduction== | ==Introduction== | ||
The gene Rv1885c from ''Mycobacteria tuberculosis'' encodes for a non-functional chorismate mutase (*MtCM)<ref name="pizza" /> | The gene Rv1885c from ''Mycobacteria tuberculosis'' encodes for a non-functional chorismate mutase (*MtCM).<ref name="pizza" /> This non-functional mutase has a 33-amino-acid cleavable sequence.<ref name="pizza" /> Chorismate mutase is a vital enzyme in the shikimate pathway, which allows for the synthesis of tryptophan, tyrosine, and phenylalanine.<ref name="pizza" /> This protein acts at the first branch point of the shikimate pathway, making it a regulating step in the conversion of prephenate from chorismate.<ref name="CMW2"> PMID:11481470 </ref> Since chorismate mutase catalyzes a claisen rearrangement it can be considered an isomerase since it catalyzes rearrangements of isomers. Chorismate mutase provides a 2x10<sup>6</sup> fold increase in the rate of reaction in comparision to the uncatalyzed reaction.<ref > P.D. Lyne, A.J. Mulholland, W.G. Richards. Insights into chorismate mutase catalysis from a combined qm/mm simulation of the enzyme reaction. Journal of the American Chemistry Society. 1995 117(45):11345-11350</ref> | ||
Chorismate mutase only occurs in bacteria, higher plants, and fungi, due to the fact that the shikimate pathway is only found in these organisms <ref name="strat" />. In ''Escherichia coli'', chorismate mutase has a periplasmic destination<ref name="pizza" />. In ''M. tuberculosis'' there is in abscence of a periplasmic compartment for chorismate mutase, so it secretes into the culture filtrate of ''M. tuberculosis''<ref name="pizza" />. It is believed that a pseudoperiplasmic space might exist in ''M. tuberculosis''<ref name="pizza" />. | Chorismate mutase only occurs in bacteria, higher plants, and fungi, due to the fact that the shikimate pathway is only found in these organisms <ref name="strat" />. In ''Escherichia coli'', chorismate mutase has a periplasmic destination<ref name="pizza" />. In ''M. tuberculosis'' there is in abscence of a periplasmic compartment for chorismate mutase, so it secretes into the culture filtrate of ''M. tuberculosis''<ref name="pizza" />. It is believed that a pseudoperiplasmic space might exist in ''M. tuberculosis''<ref name="pizza" />. | ||
