Sandbox Reserved 315: Difference between revisions
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Orotidine monophosphate decarboxylase is one of several proteins in de novo pyrimidine biosynthesis. Orotidine monophosphate (OMP) is formed when orotate reacts with 5-phosphoribosyl α-diphosphate (PRPP)<Ref name = "Brosnan">PMID:17513443</ref>. ODCase then decarboxylated OMP to form uridine monophosphate (UMP), which then goes on to be phosphorylated and converted into cytosine, uracil, and thymine<ref name = "Wu"/><ref name = "Brosnan"/>. | Orotidine monophosphate decarboxylase is one of several proteins in de novo pyrimidine biosynthesis. Orotidine monophosphate (OMP) is formed when orotate reacts with 5-phosphoribosyl α-diphosphate (PRPP)<Ref name = "Brosnan">PMID:17513443</ref>. ODCase then decarboxylated OMP to form uridine monophosphate (UMP), which then goes on to be phosphorylated and converted into cytosine, uracil, and thymine<ref name = "Wu"/><ref name = "Brosnan"/>. | ||
The mechanism for decarboxylation of OMP is still unclear. Unlike other biochemical decarboxylation reactions that stabilize the reaction intermediate through delocalizing the electron pair, OCDase shows no such stabilization<ref name = "Lee"/>. One possible mechanism is the formation of a stabilized carbene intermediate resulting from protonization at the 4-C position of OMP<Ref name = "Lee">PMID:9139656</ref>. | The mechanism for decarboxylation of OMP is still unclear. Unlike other biochemical decarboxylation reactions that stabilize the reaction intermediate through delocalizing the electron pair, OCDase shows no such stabilization<ref name = "Lee"/>. One possible mechanism is the formation of a stabilized carbene intermediate resulting from protonization at the 4-C position of OMP<Ref name = "Lee">PMID:9139656</ref>. | ||
[[Image:800px-OMPDC Reaction.png| | [[Image:800px-OMPDC Reaction.png|center|frame|Decarboxylation of OMP to UMP]] | ||
==Biological Significance== | ==Biological Significance== | ||
