User:Michael Patrick/Sandbox 2: Difference between revisions
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GFP has a typical beta barrel consisting of <scene name='User:Michael_Patrick/Sandbox_2/Ema-3/4'>one β-sheet</scene> with <scene name='User:Michael_Patrick/Sandbox_2/Ema-3/3'>alpha helix(s) containing the chromophore running through the center.</scene> Inward-facing sidechains of the barrel induce specific cyclization reactions in the <scene name='User:Michael_Patrick/Sandbox_2/Ema-3/5'>tripeptide Ser65–Tyr66–Gly67</scene> that lead to chromophore formation. This process of post-translational modification is referred to as maturation. The hydrogen-bonding network and electron-stacking interactions with these sidechains influence the color of wtGFP and its numerous derivatives. | GFP has a typical beta barrel consisting of <scene name='User:Michael_Patrick/Sandbox_2/Ema-3/4'>one β-sheet</scene> with <scene name='User:Michael_Patrick/Sandbox_2/Ema-3/3'>alpha helix(s) containing the chromophore running through the center.</scene> Inward-facing sidechains of the barrel induce specific cyclization reactions in the <scene name='User:Michael_Patrick/Sandbox_2/Ema-3/5'>tripeptide Ser65–Tyr66–Gly67</scene> that lead to chromophore formation. This process of post-translational modification is referred to as maturation. The hydrogen-bonding network and electron-stacking interactions with these sidechains influence the color of wtGFP and its numerous derivatives. | ||
<scene name='User:Michael_Patrick/Sandbox_2/Ema-3/6'>The tightly packed nature of the barrel</scene> excludes solvent molecules, protecting the chromophore fluorescence from quenching by water. | <scene name='User:Michael_Patrick/Sandbox_2/Ema-3/6'>The tightly packed nature of the barrel</scene> excludes solvent molecules, protecting the chromophore fluorescence from quenching by water. | ||
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===NOTES AND LITERATURE REFERENCES=== | |||
