DNA ligase: Difference between revisions
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[[Image:2hix.png|left|200px|thumb|Crystal Structure of DNA ligase [[2hix]]]] | [[Image:2hix.png|left|200px|thumb|Crystal Structure of DNA ligase [[2hix]]]] | ||
{{STRUCTURE_2hix| PDB=2hix | SIZE=300| | {{STRUCTURE_2hix| PDB=2hix | SIZE=300| SCENE=DNA_ligase/Cv/1 |right|CAPTION=ATP-dependent DNA ligase [[2hix]] }} | ||
'''DNA ligase''' (LigD) is an enzyme which repairs single-stranded breaks in a double-stranded DNA. LigD is activated , in a species-dependent manner, by hydrolysis of ATP or NAD+. Mamlian LigD I ligates the nascent DNA of the lagging strand; LigD III complexes with XRCC1 in the process of nucleotide excision repair; LigD IV complexes with XRCC4 and catalyzes the last step in the non-homologous DNA end joining. | '''DNA ligase''' (LigD) is an enzyme which repairs single-stranded breaks in a double-stranded DNA. LigD is activated , in a species-dependent manner, by hydrolysis of ATP or NAD+. Mamlian LigD I ligates the nascent DNA of the lagging strand; LigD III complexes with XRCC1 in the process of nucleotide excision repair; LigD IV complexes with XRCC4 and catalyzes the last step in the non-homologous DNA end joining. | ||
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[[1l7b]] – LigD BRCT domain – ''Thermus thermophilus'' - NMR | [[1l7b]] – LigD BRCT domain – ''Thermus thermophilus'' - NMR | ||
=== | ===Mammalian LigD I=== | ||
[[1x9n]] – hLigD I + DNA + AMP + CMP – human | [[1x9n]] – hLigD I + DNA + AMP + CMP – human | ||
=== | ===Mammalian LigD III=== | ||
[[3l2p]] – hLigD III residues 257-833 + DNA<br /> | [[3l2p]] – hLigD III residues 257-833 + DNA<br /> | ||
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[[1imo]], [[ 1in1]] - hLigD III BRCTdomain – NMR | [[1imo]], [[ 1in1]] - hLigD III BRCTdomain – NMR | ||
=== | ===Mammalian LigD VI=== | ||
[[3ii6]], [[ 1ik9]] – hLigD IV C-terminal BRCT domains + DNA repair protein XRCC4<br /> | [[3ii6]], [[ 1ik9]] – hLigD IV C-terminal BRCT domains + DNA repair protein XRCC4<br /> | ||
