TolB: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 6: | Line 6: | ||
TolB is a 44-kDa periplasmic protein associated with the outer membrane. It has two domains: an N-terminal α/β domain and a C-terminal six-bladed β-propeller (to which [[Pal]] and [[Colicin E9]] bind) <ref>PMID: 19696740</ref>. The β-propeller has a latching or ‘Velco’ strand which joins the first and last of the six blades, and is positioned in the domain-domain interface. When Pal binds to the C-terminus of TolB, the latching strand moves away from the interface and carries with it a proline residue. The movement of the latching strand opens up a canyon that would normally be buried between the N- and C-terminal domains of TolB. This canyon can now be used as a binding site for the N-terminal of TolB, which forms a helical half-turn and a β-sheet against the canyon. | TolB is a 44-kDa periplasmic protein associated with the outer membrane. It has two domains: an N-terminal α/β domain and a C-terminal six-bladed β-propeller (to which [[Pal]] and [[Colicin E9]] bind) <ref>PMID: 19696740</ref>. The β-propeller has a latching or ‘Velco’ strand which joins the first and last of the six blades, and is positioned in the domain-domain interface. When Pal binds to the C-terminus of TolB, the latching strand moves away from the interface and carries with it a proline residue. The movement of the latching strand opens up a canyon that would normally be buried between the N- and C-terminal domains of TolB. This canyon can now be used as a binding site for the N-terminal of TolB, which forms a helical half-turn and a β-sheet against the canyon. | ||
TolB forms a complex with Pal (see | TolB forms a complex with Pal ('see below') which plays a role in maintaining the integrity of the outer membrane. | ||
==Function== | ==Function== | ||
| Line 13: | Line 13: | ||
==The TolB-Pal Complex== | ==The TolB-Pal Complex== | ||
{{STRUCTURE_2hqs | PDB=2hqs | SCENE= TolB-Pal_Complex/Tolbpalmulticomplex/1 }} | {{STRUCTURE_2hqs | PDB=2hqs | SCENE= TolB-Pal_Complex/Tolbpalmulticomplex/1 }} | ||
The | The TolB-[[Pal]] complex is involved in maintaining the outer membrane integrity. This complex is parasitised by protein antibiotics and disrupted in order to trigger the translocation of the toxin across the outer membrane (see [[Colicin]] for further information).<ref name='Bonsor'>PMID: 17375930</ref> | ||
==Related Tol entries== | ==Related Tol entries== | ||
