Tol: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:TOL.jpg|400px|right|thumb| The Tol System<ref name='Witty'> PMID: 12169623</ref> and Operon<ref>http://ecoliwiki.net/colipedia/index.php/tolA:Expression</ref><ref>http://ecoliwiki.net/colipedia/index.php/tolB:Expression</ref> ]] | [[Image:TOL.jpg|400px|right|thumb| The Tol System<ref name='Witty'> PMID: 12169623</ref> and Operon<ref>http://ecoliwiki.net/colipedia/index.php/tolA:Expression</ref><ref>http://ecoliwiki.net/colipedia/index.php/tolB:Expression</ref> ]] | ||
==Structure== | ==Structure== | ||
| Line 12: | Line 11: | ||
==Interaction of Tol with Colicins== | ==Interaction of Tol with Colicins== | ||
A yeast two-hybrid screen was carried out in order to determine the interactions between colicins and the Tol-Pal system during the import of colicin.<ref>PMID: 11994151<ref/> The screen showed that TolB dimerizes, and its amino terminal domain (D1) interacts with the periplasmic, C-terminal domain of TolA (TolAIII), whilst the central domain of TolA (TolAII) interacts with [[YbgF]]. It is the interaction between TolAIII and D1 that forms a "''trans''-envelope complex" which brings the inner and outer membranes closer together allowing for the uptake of colicin A. | A yeast two-hybrid screen was carried out in order to determine the interactions between colicins and the Tol-Pal system during the import of colicin.<ref>PMID: 11994151<ref/> The screen showed that TolB dimerizes, and its amino terminal domain (D1) interacts with the periplasmic, C-terminal domain of TolA (TolAIII), whilst the central domain of TolA (TolAII) interacts with [[YbgF]]. It is the interaction between TolAIII and D1 that forms a "''trans''-envelope complex" which brings the inner and outer membranes closer together allowing for the uptake of colicin A. | ||
| Line 22: | Line 19: | ||
==Regulation== | ==Regulation== | ||
It has been shown that the regulation of Tol genes is linked to the cell envelope stability. <ref>PMID: 17347522</ref> In studies with E. coli, the ''tol-pal'' genes have been induced by RcsC in response to cell envelope stress. <ref>PMID: 8821933</ref> RcsC is a transmembrane sensor kinase, and along with the cytoplasmic response regulator RcsB, makes up the RcsBC regulatory system. <ref>PMID: 16166540</ref> RcsBC is involved in the regulation of ''cps'' genes which code for the biosynthsis machinery of a major component for the capsula, cholanic acid. | It has been shown that the regulation of Tol genes is linked to the cell envelope stability. <ref>PMID: 17347522</ref> In studies with E. coli, the ''tol-pal'' genes have been induced by RcsC in response to cell envelope stress. <ref>PMID: 8821933</ref> RcsC is a transmembrane sensor kinase, and along with the cytoplasmic response regulator RcsB, makes up the RcsBC regulatory system. <ref>PMID: 16166540</ref> RcsBC is involved in the regulation of ''cps'' genes which code for the biosynthsis machinery of a major component for the capsula, cholanic acid. | ||
== References== | == References== | ||
<references/> | <references/> | ||
