Tol: Difference between revisions
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A yeast two-hybrid screen was carried out in order to determine the interactions between colicins and the Tol-Pal system during the import of colicin.<ref name="Walburger">PMID: 11994151</ref> The screen showed that TolB dimerizes, and its amino terminal domain (D1) interacts with the periplasmic, C-terminal domain of TolA (TolAIII), whilst the central domain of TolA (TolAII) interacts with [[YbgF]]. It is the interaction between TolAIII and D1 that forms a "''trans''-envelope complex" which brings the inner and outer membranes closer together allowing for the uptake of colicin A. The N-terminal of the group A colicins then interact with TolA and also sometimes TolB during translocation into the inner membrane. | A yeast two-hybrid screen was carried out in order to determine the interactions between colicins and the Tol-Pal system during the import of colicin.<ref name="Walburger">PMID: 11994151</ref> The screen showed that TolB dimerizes, and its amino terminal domain (D1) interacts with the periplasmic, C-terminal domain of TolA (TolAIII), whilst the central domain of TolA (TolAII) interacts with [[YbgF]]. It is the interaction between TolAIII and D1 that forms a "''trans''-envelope complex" which brings the inner and outer membranes closer together allowing for the uptake of colicin A. The N-terminal of the group A colicins then interact with TolA and also sometimes TolB during translocation into the inner membrane. | ||
The structure shows the interaction between [[TolB]] and the translocation domain of [[Colicin E9]]. | The structure shows the interaction between [[TolB]] and the <scene name='Tol/Tolbcolclose/1'>translocation domain</scene> of [[Colicin E9]]. | ||
==Regulation== | ==Regulation== | ||
It has been shown that the regulation of Tol genes is linked to the cell envelope stability.<ref>PMID: 17347522</ref> In studies with E. coli, the ''tol-pal'' genes have been induced by RcsC in response to cell envelope stress.<ref>PMID: 8821933</ref> RcsC is a transmembrane sensor kinase, and along with the cytoplasmic response regulator RcsB, makes up the RcsBC regulatory system.<ref>PMID: 16166540</ref> RcsBC is involved in the regulation of ''cps'' genes which code for the biosynthsis machinery of a major component for the capsula, cholanic acid. | It has been shown that the regulation of Tol genes is linked to the cell envelope stability.<ref>PMID: 17347522</ref> In studies with E. coli, the ''tol-pal'' genes have been induced by RcsC in response to cell envelope stress.<ref>PMID: 8821933</ref> RcsC is a transmembrane sensor kinase, and along with the cytoplasmic response regulator RcsB, makes up the RcsBC regulatory system.<ref>PMID: 16166540</ref> RcsBC is involved in the regulation of ''cps'' genes which code for the biosynthsis machinery of a major component for the capsula, cholanic acid. | ||
