Sandbox20: Difference between revisions
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===Role=== | ===Role=== | ||
Tus is the 36 kDa protein responsible for termination of replication in Escherichia coli. The chromosome of E. coli contains a set of six polar Ter DNA sequences arranged such that three with the same directionality are located on either half of the chromosome. This generates what is termed a replication-fork trap, which prevents replication from occurring towards the origin. The Ter sites contain a 20 bp consensus element to which a monomer of Termination Utilisation Substance (Tus) binds to form a polar DNA-protein complex which halts the progression of the replicative machinery from one direction only. The fork arrest mechanism depends on the blocking of the helicase activity of DnaB, which is the first component of the replisome to encounter the Ter- | Tus is the 36 kDa protein responsible for termination of replication in Escherichia coli. The chromosome of E. coli contains a set of six polar Ter DNA sequences arranged such that three with the same directionality are located on either half of the chromosome. This generates what is termed a replication-fork trap, which prevents replication from occurring towards the origin. The Ter sites contain a 20 bp consensus element to which a monomer of Termination Utilisation Substance (Tus) binds to form a polar DNA-protein complex which halts the progression of the replicative machinery from one direction only. The fork arrest mechanism depends on the blocking of the helicase activity of DnaB, which is the first component of the replisome to encounter the Ter-Tus complex. | ||
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===Structural Overview=== | ===Structural Overview=== | ||
