Chitinase: Difference between revisions

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[[Chitinases]] cleave the beta-1-4-glycosidic bond between the N-acetyl-D-glucosamine units of which chitin is comprised. Chitinases are present in plants, bacteria and fungi. The first chitinase structures were solved in 1994, from a bacterium ([[1ctn]]) and a plant ([[2hvm]]). A mechanism for chitin cleavage was proposed based on several structures and was later confirmed.
[[Chitinases]] cleave the beta-1-4-glycosidic bond between the N-acetyl-D-glucosamine units of which chitin is comprised. Chitinases are present in plants, bacteria and fungi. The first chitinase structures were solved in 1994, from a bacterium ([[1ctn]]) and a plant ([[2hvm]]). A mechanism for chitin cleavage was proposed based on several structures and was later confirmed. The images at the left and at the right correspond to one representative Chitinase, ''i.e.'' the crystal structure of Chitinase I from ''Serratia marcescens'' complex with inhibitor ([[1ffq]]).
 
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== 3D Structures of Chitinase ==
 
===Chitinase I===
 
[[3aro]], [[3b8s]] – VhChi residues 22-597 – ''Vibrio harveyi''<br />
[[3b9e]] - VhChi residues 22-597 (mutant)<br />
[[1k85]] – BcChi fibronectin domain – ''Bacillus circulans'' – NMR<br />
[[1itx]] - BcChi catalytic domain<br />
[[1ed7]] – BcChi chitin-binding domain - NMR<br />
[[1ll6]], [[1ll7]] - CiChi residues 36-427 (mutant) – ''Coccicioides immitis'' <br />
[[1d2k]] - CiChi residues 36-427<br />
[[3n11]] – BcChi – ''Bacillus cereus''<br />
[[3iwr]], [[2dkv]] – Chi residues 33-340 – Japanese rice<br />
[[1edq]], [[1ctn]] - SmChi – ''Serratia marcescens''<br />
[[1x6l]], [[1rd6]] – SmChi (mutant)<br />
[[3g6l]] - BoChi residues 21-426 – B''ionectria ochroleuca''<br />
 
====Chitinase I binary complex====
 
[[3arp]], [[3arq]], [[3arr]], [[3ars]], [[3art]], [[3aru]], [[3arv]], [[3arw]], [[3arx]], [[3ary]], [[3arz]] – VhChi residues 22-597 + inhibitor
[[3as0]], [[3as1]], [[3as2]], [[3as3]] – VhChi residues 22-597 (mutant) + inhibitor
[[3b9a]], [[3b9d]] - VhChi residues 22-597 + polysaccharide
2xvn, 2xuc – AfChi residues 29-307 + inhibitor – Aspergillus fumigatus
3n13, 3n15, 3n17, 3n18 – BcChi (mutant) + NAG
3n12 – BcChi + Zn
3n1a - BcChi (mutant) + inhibitor
1x6n - SmChi (mutant) + inhibitor
1ffq - SmChi + inhibitor
1nh6, 1k9t, 1ffr, 1eib, 1ehn - SmChi (mutant) + polysaccharide
2wk2, 2wly, 2wlz, 2wm0 – SmChi residues 2-520 + inhibitor
3g6m - BoChi residues 21-426 + caffeine
1waw, 1wb0 – hChi + polypeptide – human
1hkk, 1hkm – hChi + inhibitor
1ll4 - CiChi residues 36-427 + inhibitor
 
===Chitinase II===
 
1e15 - SmChi
1ogb, 1goi, 1e6p - SmChi (mutant)
1kfw – Chi catalytic domain – Arthrobacter
1dxj – Chi – Jack bean
 
====Chitinase II binary complex====
 
1ogg, 1ur9 - SmChi (mutant) + inhibitor
1e6n - SmChi (mutant) + polysaccharide
1ur8, 1gpf, 1e6r - SmChi + inhibitor
1e6z – SmChi + intermediate
2a3a, 2a3b, 2a3c, 2a3e – AfChi + inhibitor
1w1p, 1w1t, 1w1v, 1w1y, 1o6i, 1h0g, 1h0i – SmChi + polypeptide
 
===Chitinase III===
 
2dbt, 2wvu – SgChi residues 30-294 – Streptomyces griseus
1wvv - SgChi residues 30-294 (mutant)
2d49 – SgChi chitin-binding domain - NMR
 
===Chitinase IV===
 
3hbd, 3hbe, 3hbh - Chi catalytic domain – Norway spruce
 
===Chitinase V===
 
3alf – tChi residues 26-377 – tobacco
 
====Chitinase V binary complex====
 
3alg - tChi residues 26-377 (mutant) + NAG
3chc, 3chd, 3che, 3chf - AfChi + polypeptide
3ch9, 2iuz – AfChi + inhibitor
 
===Chitinase VIII===
 
2cjl - Chi residues 41-244 – Streptomyces coelicolor
 
===Chitinase===
 
1wno, 1w9p - AfChi
2dsk - PfChi catalytic domain – Pyrococcus furiosus
3afb - PfChi catalytic domain (mutant)
1cns – Chi – Hordeum vulgare
2czn - PfChi chitin-binding domain – NMR
2cwr - PfChi chitin-binding domain
3ian – Chi (mutant) – Lactococcus lactis
3fxy - hChi catalytic domain
1lq0 - hChi residues 22-286
1guv - hChi residues 22-387
3fnd, 3co4 – Chi – Bacteroides thetaiotamicron
3cql – Chi – Carica papaya
2z37 - BjChi catalytic domain – Brassica juncea
2z39 – BjChi catalytic domain (mutant)
2uy2 – yChi residues 22-315 – yeast
 
====Chitinase  binary complex====
 
3a4w, 3a4x – PfChi catalytic domain (mutant) + NAG
1lg1 - hChi residues 22-387 + chitobiose
1lg2 - hChi residues 22-387 + ethylene glycol
3fy1 - hChi catalytic domain + inhibitor
1hki, 1hkj - hChi + inhibitor
2z38 - BjChi catalytic domain + Cl
2uy3, 2uy4, 2uy5 - yChi residues 22-315 + inhibitor
1w9v, 1w9u - AfChi + polypeptide
 
 
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