Bacterial Replication Termination: Difference between revisions

<StructureSection load='2ewj' size='500' side='left' caption= 'Tus complexed to the ''E. coli'' ''ter'' site' scene=''>The ''E.coli'' protein that is responsible for termination is a 36kDa protein named Tus (Terminius Utilization Substance) that binds 23bp ''ter'' sites and arrests the replication helicase, DnaB, responsible for separating the two strands of DNA []. Unlike RTP termination sites, the ten ''E.coli'' ''ter'' sites do not contain inverted sequences or direct repeats and Tus binds as a monomer to a highly conserved core region of 13bp [8]. The tus-''ter'' complex is known to terminate replication by arresting the replication machinery in a in a polar manner however there is great discrepancy in evidence whether Tus specifically interacts or physically blocks the DnaB helicase to arrest its progression [1].