User:Cameron Ball/Sandbox 1: Difference between revisions
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==The Termination Sites== | ==The Termination Sites== | ||
[[Image:Ter_site_DNA.png|300px|right|thumb| DNA sequence of a Ter site showing the A-site in Red, the B-site in Blue and the 3 base pair overlap in purple]] | |||
To arrest DNA replication, the two RTP dimers bind at a specific DNA site. These sites, christened ''Ter'' sites, are 29 base pairs in length and comprise of two non-identical inverted repeats that share three highly conserved base pairs. The two sites are designated the A-site and the B-site and each have different affinities for RTP (with the B-site exhibiting greater protein-DNA contact and thus greater affinity<ref>Langley, D. B., Smith, M. T., Lewis, P. J., and Wake, R. G. Protein-nucleoside contacts in the interaction between the replication terminator protein of Bacillus subtilis and the DNA terminator. (1993) Mol. Microbiol. 10, 771-779</ref>). Each site binds one dimer of RTP cooperatively, with the higher affinity B-site being bound first, then the A-site. <ref>A.V. Kralicek, P.K. Wilson, G.B. Ralston, R.G. Wake and G.F. King, Reorganization of terminator DNA upon binding replication terminator protein: implications for the functional replication fork arrest complex, Nucl. Acids Res. 25 (1997), pp. 590–596</ref> | To arrest DNA replication, the two RTP dimers bind at a specific DNA site. These sites, christened ''Ter'' sites, are 29 base pairs in length and comprise of two non-identical inverted repeats that share three highly conserved base pairs. The two sites are designated the A-site and the B-site and each have different affinities for RTP (with the B-site exhibiting greater protein-DNA contact and thus greater affinity<ref>Langley, D. B., Smith, M. T., Lewis, P. J., and Wake, R. G. Protein-nucleoside contacts in the interaction between the replication terminator protein of Bacillus subtilis and the DNA terminator. (1993) Mol. Microbiol. 10, 771-779</ref>). Each site binds one dimer of RTP cooperatively, with the higher affinity B-site being bound first, then the A-site. <ref>A.V. Kralicek, P.K. Wilson, G.B. Ralston, R.G. Wake and G.F. King, Reorganization of terminator DNA upon binding replication terminator protein: implications for the functional replication fork arrest complex, Nucl. Acids Res. 25 (1997), pp. 590–596</ref> | ||
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''B.subtilis'' has multiple Ter sites of both polarities to ensure that replication is terminated. The sites are situated off center in relation to the OriC to provide some redundancy at the recombination site. This ensures the entire genome is copied faithfully. | ''B.subtilis'' has multiple Ter sites of both polarities to ensure that replication is terminated. The sites are situated off center in relation to the OriC to provide some redundancy at the recombination site. This ensures the entire genome is copied faithfully. | ||
== Structure== | == Structure== | ||
