User:Nathan Harris/Tus: Difference between revisions
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with Tus leading to dissociation of Tus and progression of the replication fork. | with Tus leading to dissociation of Tus and progression of the replication fork. | ||
However, when ''E. coli'' ''Ter'' sequences are inserted into a plasmid in ''B. Subtillis'' expressing Tus, the replication fork arrest from the non-permissive | However, when ''E. coli'' ''Ter'' sequences are inserted into a plasmid in ''B. Subtillis'' expressing Tus, the replication fork arrest from the non-permissive | ||
end only occurs with 0.5% efficiency compared to 45.4% efficiency in a wild type ''E. coli'' system<ref name = "Anderson"> Anderson, P., Griffith, A., Duggin, I. and Wake, R. (2000) Functional specificity of the replication fork-arrest complexes of Bacillus subtilis and Escherichia coli: significant specificity for Tus-Ter functioning in E.coli. Molecular Microbiology, 36 (6): 1327-1335.</ref>. If only Tus-''Ter'' interactions were important in the | end only occurs with 0.5% efficiency compared to 45.4% efficiency in a wild type ''E. coli'' system <ref name = "Anderson"> Anderson, P., Griffith, A., Duggin, I. and Wake, R. (2000) Functional specificity of the replication fork-arrest complexes of Bacillus subtilis and Escherichia coli: significant specificity for Tus-Ter functioning in E.coli. Molecular Microbiology, 36 (6): 1327-1335.</ref>. If only Tus-''Ter'' interactions were important in the | ||
mediation of polar fork arrest, then the efficiency in the two systems should be similar. This highlights the importance of other factors in the | mediation of polar fork arrest, then the efficiency in the two systems should be similar. This highlights the importance of other factors in the | ||
mediation of polar fork arrest. | mediation of polar fork arrest. | ||
